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Abstract <p>The objective of this study is to utilize novel hybrid biomaterials, comprising glucose oxidase coated with an alkyl-modified silica polymer shell in conjunction with a Clark oxygen electrode, as biocatalysts. This work studied the efficiency of immobilization of glucose oxidase in a sol-gel matrix based on the silane precursors tetraethoxysilane, methyltriethoxysilane, and diethoxydimethylsilane. The use of a 50/50 vol % mixture of methyltriethoxysilane and tetraethoxysilane produces a polymer material with smaller pores compared to a mixture of other silane precursors. When precursors are added during the formation of the material with non-hydrolyzed bonds, the surface area of the materials increases from 40 m<sup>2</sup>/g when using only tetraethoxysilane to 70 m<sup>2</sup>/g with a mixture of diethoxydimethylsilane and tetraethoxysilane, and up to 110 m<sup>2</sup>/g with a mixture of methyltriethoxysilane and tetraethoxysilane. In addition, adding diethoxydimethylsilane to the tetraethoxysilane system increases the pore volume from 0.008 to 0.09 cm<sup>3</sup>/g, and adding methyltriethoxysilane increases the pore volume to 0.33 cm<sup>3</sup>/g. A biosensor using glucose oxidase immobilized in a polymer matrix based on a diethoxydimethylsilane/tetraethoxysilane 50/50 vol % mixture is characterized by a high sensitivity coefficient of 3.8 mg O<sub>2</sub>/min mmol and a glucose concentration range of 1–280 mmol/L. Therefore, this study demonstrates that the use of polymers with linear fragments is more effective for the immobilization of glucose oxidase compared to traditional immobilization methods. This could lead to the development of new and efficient enzyme immobilization methods for the determination of individual substances in biological fluids, e.g., blood glucose.</p>

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Efficient Approach to the Immobilization of Glucose Oxidase in Sol-Gel Polymeric Matrices Using Silane Precursors with One and Two Non-Hydrolyzable Bonds

  • 期刊论文

  • 发表日期：

    2025年7月25日

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  • O. A. Kamanina,
  • E. A. Lantsova,
  • P. V. Rybochkin

摘要

Abstract

The objective of this study is to utilize novel hybrid biomaterials, comprising glucose oxidase coated with an alkyl-modified silica polymer shell in conjunction with a Clark oxygen electrode, as biocatalysts. This work studied the efficiency of immobilization of glucose oxidase in a sol-gel matrix based on the silane precursors tetraethoxysilane, methyltriethoxysilane, and diethoxydimethylsilane. The use of a 50/50 vol % mixture of methyltriethoxysilane and tetraethoxysilane produces a polymer material with smaller pores compared to a mixture of other silane precursors. When precursors are added during the formation of the material with non-hydrolyzed bonds, the surface area of the materials increases from 40 m2/g when using only tetraethoxysilane to 70 m2/g with a mixture of diethoxydimethylsilane and tetraethoxysilane, and up to 110 m2/g with a mixture of methyltriethoxysilane and tetraethoxysilane. In addition, adding diethoxydimethylsilane to the tetraethoxysilane system increases the pore volume from 0.008 to 0.09 cm3/g, and adding methyltriethoxysilane increases the pore volume to 0.33 cm3/g. A biosensor using glucose oxidase immobilized in a polymer matrix based on a diethoxydimethylsilane/tetraethoxysilane 50/50 vol % mixture is characterized by a high sensitivity coefficient of 3.8 mg O2/min mmol and a glucose concentration range of 1–280 mmol/L. Therefore, this study demonstrates that the use of polymers with linear fragments is more effective for the immobilization of glucose oxidase compared to traditional immobilization methods. This could lead to the development of new and efficient enzyme immobilization methods for the determination of individual substances in biological fluids, e.g., blood glucose.