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Abstract <p>Silver nanoparticles (AgNPs–E) biosynthesized at room and boiling <i>T</i>, stabilized by blackberry plant extracts (E) obtained by maceration at room <i>T</i> and reflux extraction at boiling <i>T</i>, are presented in this work. The obtained AgNPs–E were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS) and zeta-potential measurement. XRD analysis confirmed that the 2θ peak at 38.2°, corresponding to the (111) plane, was the most intense, with the average crystallite size in the range of 18.0 ± 3–21.1 ± 2 nm. SEM spectroscopy showed that spherical shapes of nanoparticles dominate in the reaction mixture. EDX spectroscopy showed the presence of elemental silver in the formed AgNPs–E, along with the presence of C and Mg, which probably originate from the biomolecules of the extracts. DLS provides the size distribution with the average particle size in the range of 50.93 ± 0.83–74.48 ± 1.84 nm, with negative zeta-potential ranged from –25.87 ± 1.63 to –0.3027 ± 0.36 mV indicating the AgNPs stability. The antimicrobial activity of AgNPs–E was tested by microdilution method confirming that both nanoparticles and extracts show the ability to inhibit all the bacteria and a fungus tested. The presented results suggest further investigations of synthesized AgNPs–E for application in topical cosmetic preparations.</p>

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Characterization and Biological Activity of Silver Nanoparticles Stabilized by Aqueous Extracts of Rubusspp. Leaves

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • Marija S. Tasić,
  • Jelena B. Zvezdanović,
  • Ljiljana P. Stanojević,
  • Jelena S. Stanojević,
  • Sanja M. Petrović,
  • BojanaDanilović,
  • Dragan J. Cvetković

摘要

Abstract

Silver nanoparticles (AgNPs–E) biosynthesized at room and boiling T, stabilized by blackberry plant extracts (E) obtained by maceration at room T and reflux extraction at boiling T, are presented in this work. The obtained AgNPs–E were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS) and zeta-potential measurement. XRD analysis confirmed that the 2θ peak at 38.2°, corresponding to the (111) plane, was the most intense, with the average crystallite size in the range of 18.0 ± 3–21.1 ± 2 nm. SEM spectroscopy showed that spherical shapes of nanoparticles dominate in the reaction mixture. EDX spectroscopy showed the presence of elemental silver in the formed AgNPs–E, along with the presence of C and Mg, which probably originate from the biomolecules of the extracts. DLS provides the size distribution with the average particle size in the range of 50.93 ± 0.83–74.48 ± 1.84 nm, with negative zeta-potential ranged from –25.87 ± 1.63 to –0.3027 ± 0.36 mV indicating the AgNPs stability. The antimicrobial activity of AgNPs–E was tested by microdilution method confirming that both nanoparticles and extracts show the ability to inhibit all the bacteria and a fungus tested. The presented results suggest further investigations of synthesized AgNPs–E for application in topical cosmetic preparations.