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Abstract <p>The promoter-operator region of the <i>Bacillus cereus hlyII</i> gene includes an elongated operator region with mirror symmetry, recognizable with the main specific transcriptional regulator HlyIIR. In addition, regions for the global transcription regulators Fur, OhrR, and ResD are located in the region of the <i>hlyII</i> gene operator. The latter is a transcriptional regulator of the redox-sensitive ResDE signal transduction pathway. <i>Bacillus cereus</i> sensu lato strains were found with a disturbance in the proximal part of the area recognized by HlyIIR and ResD. The essential role of these regions in the expression of the <i>hlyII</i> gene has been demonstrated. Natural strains of <i>Bacillus cereus</i> with deletions in the proximal region of the HlyIIR operator of the <i>hlyII</i> gene that have a significantly reduced level of expression of <i>hlyII</i> were identified. Disturbances in the HlyIIR operator reduce the expression of <i>hlyII</i> by several tens of times. The presence of an intact recognition site for ResD reduces the expression of this gene by several times under aerobic conditions. These results allow us to determine the influence of structural variability in the promoter-operator region of <i>Bacillus cereus hlyII</i> genes on its transcriptional activity.</p>

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Variability of the Promoter-Operator Region of Bacillus cereus hlyII Gene Impacts on Its Transcriptional Activity Level

  • 期刊论文

  • 发表日期：

    2025年6月26日

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  • A. M. Shadrin,
  • E. V. Shapyrina,
  • A. S. Nagel,
  • A. V. Siunov,
  • Zh. I. Andreeva-Kovalevskaya,
  • V. I. Salyamov,
  • A. S. Solonin

摘要

Abstract

The promoter-operator region of the Bacillus cereus hlyII gene includes an elongated operator region with mirror symmetry, recognizable with the main specific transcriptional regulator HlyIIR. In addition, regions for the global transcription regulators Fur, OhrR, and ResD are located in the region of the hlyII gene operator. The latter is a transcriptional regulator of the redox-sensitive ResDE signal transduction pathway. Bacillus cereus sensu lato strains were found with a disturbance in the proximal part of the area recognized by HlyIIR and ResD. The essential role of these regions in the expression of the hlyII gene has been demonstrated. Natural strains of Bacillus cereus with deletions in the proximal region of the HlyIIR operator of the hlyII gene that have a significantly reduced level of expression of hlyII were identified. Disturbances in the HlyIIR operator reduce the expression of hlyII by several tens of times. The presence of an intact recognition site for ResD reduces the expression of this gene by several times under aerobic conditions. These results allow us to determine the influence of structural variability in the promoter-operator region of Bacillus cereus hlyII genes on its transcriptional activity.