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<b>Abstract</b>— <p>For 18 <i>Pyrenophora tritici-repentis</i> strains, the pathogenic micromycete causing tan spot of wheat, the race was determined and the <i>ToxB/toxb</i> genes were identified. The analyzed strains belonged predominantly to race 4, which is nonpathogenic for bread wheat. Alignment of sequences of the <i>ToxB/toxb</i> gene from one <i>P. tritici-repentis</i> strain from Kazakhstan and five strains from Tatarstan with the reference sequences of the <i>ToxB1</i>, <i>toxb2</i>, <i>ToxB4</i>, <i>toxb12</i>, and <i>toxb14</i> genes allowed to accurately identify the genes analyzed in this study as <i>toxb2</i>. This is the first report of the <i>toxb2</i> gene in <i>P. tritici-repentis</i> populations from Russia and Kazakhstan. Also, differences between the <i>ToxB1</i> and <i>toxb2</i> sequences were demonstrated: 27 nucleotide substitutions and one 3-bp deletion were found in the ORF region in <i>ToxB1</i>. In the 5'UTR region of all obtained <i>toxb2</i> sequences, as well as in reference <i>ToxB1</i> sequences, the presence of four microsatellites (25 bp each) was detected between the TATA-box and the intron. In the <i>toxb2</i> sequences of reference <i>P. tritici-repentis</i> strain SD20 and analyzed strains, a 167-bp insertion was found in the 5'UTR region before the intron. This insertion is not found in any known <i>ToxB1</i> sequence and likely affects the functionality of the <i>toxb2</i> gene. Novel information on structure of the <i>toxb2</i> gene has implications for understanding the evolution of <i>ToxB/toxb</i> genes of <i>P. tritici-repentis</i>.</p>

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First Detection of the toxb2 Gene in Pyrenophora tritici-repentis Strains from Russia and Kazakhstan

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • N. V. Mironenko,
  • A. S. Orina,
  • N. M. Kovalenko

摘要

Abstract—

For 18 Pyrenophora tritici-repentis strains, the pathogenic micromycete causing tan spot of wheat, the race was determined and the ToxB/toxb genes were identified. The analyzed strains belonged predominantly to race 4, which is nonpathogenic for bread wheat. Alignment of sequences of the ToxB/toxb gene from one P. tritici-repentis strain from Kazakhstan and five strains from Tatarstan with the reference sequences of the ToxB1, toxb2, ToxB4, toxb12, and toxb14 genes allowed to accurately identify the genes analyzed in this study as toxb2. This is the first report of the toxb2 gene in P. tritici-repentis populations from Russia and Kazakhstan. Also, differences between the ToxB1 and toxb2 sequences were demonstrated: 27 nucleotide substitutions and one 3-bp deletion were found in the ORF region in ToxB1. In the 5'UTR region of all obtained toxb2 sequences, as well as in reference ToxB1 sequences, the presence of four microsatellites (25 bp each) was detected between the TATA-box and the intron. In the toxb2 sequences of reference P. tritici-repentis strain SD20 and analyzed strains, a 167-bp insertion was found in the 5'UTR region before the intron. This insertion is not found in any known ToxB1 sequence and likely affects the functionality of the toxb2 gene. Novel information on structure of the toxb2 gene has implications for understanding the evolution of ToxB/toxb genes of P. tritici-repentis.