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<p><b>Abstract</b>—Using the specimens of the domestic yak and three cattle breeds, the differentiating potential of Chr4:68609356G&gt;T (<i>JAZF1</i> gene), Chr14:35695388G&gt;T (<i>SLCO5A1</i> gene), and Chr19:63181970C&gt;G (<i>CEP112</i> gene) polymorphic variants was assessed. High accuracy (99.67%), specificity (100%), and sensitivity (100%) of the suggested test model, consisting of these three polymorphisms for identification of the domestic yak and cattle, were confirmed. A fast and simple identification method based on this model using kompetitive allele-specific PCR (KASP) technology was developed, which made it possible to considerably reduce the time and cost of molecular genetic analysis, as well as to reduce the risk of cross-contamination of the specimens.</p>

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Identification of Bos taurus and Bos grunniens Using SNPs

  • 期刊论文

  • 发表日期：

    2025年1月1日

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  • V. N. Kipen,
  • Zh. T. Isakova,
  • M. M. Patrin,
  • K. B. Chekirov,
  • K. A. Aitbaev,
  • A. R. Karypova,
  • M. I. Irsaliev

摘要

Abstract—Using the specimens of the domestic yak and three cattle breeds, the differentiating potential of Chr4:68609356G>T (JAZF1 gene), Chr14:35695388G>T (SLCO5A1 gene), and Chr19:63181970C>G (CEP112 gene) polymorphic variants was assessed. High accuracy (99.67%), specificity (100%), and sensitivity (100%) of the suggested test model, consisting of these three polymorphisms for identification of the domestic yak and cattle, were confirmed. A fast and simple identification method based on this model using kompetitive allele-specific PCR (KASP) technology was developed, which made it possible to considerably reduce the time and cost of molecular genetic analysis, as well as to reduce the risk of cross-contamination of the specimens.