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Abstract <p>Germination and subsequent growth stages of sesame is often affected by drought and waterlogging stresses, respectively. With an aim to identify genotypes possessing stage specific water stress responses, laboratory and pot culture experiments were envisaged. In laboratory condition, fifteen sesame genotypes were subjected to polyethylene glycol (PEG) induced osmotic stress. Percentage of normal seedlings, root length, shoot length and vigour index I (VI) were observed to be negatively affected under PEG induced osmotic stress, whereas the proportion of abnormal seedlings and dead seeds increased. The same set of genotypes were subjected to waterlogging stress for a period of 72 h at vegetative stage in a pot culture. Based on biochemical and morphological performances across both stresses, genotypes were grouped into different clusters with a target to identify the ones with seedling level osmotic stress tolerance and vegetative stage waterlogging stress tolerance. The group of tolerant genotypes possessed higher survival rate and recovery response. Reduction in shoot and root dry weights, and plant height were minimal in the tolerant ones compared to susceptible genotypes. They also demonstrated an increase in root number in contrast to susceptible ones. Reactive oxygen species (ROS) production was higher in susceptible genotypes in terms of increased malondialdehyde (MDA) content while the catalase enzyme activity was lesser in contrast to the tolerant genotypes. Z&#xa0;scatter analysis of important morphological parameters under waterlogging and osmotic stress conditions revealed that the wild genotype <i>Sesamum malabaricum</i> possessed tolerance to both stresses, whereas CO 1 was susceptible to both stresses. Sequencing of <i>XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE</i> gene in these contrasting genotypes revealed sequence dissimilarity. <i>In silico</i> protein analysis revealed a change in domain constitution in the xyloglucan endotransglucosylase/hydrolase in the susceptible genotype compared to the tolerant one. Molecular docking studies revealed a reduction in cavity size of the enzyme in the susceptible genotype which plausibly affects its functionality.</p>

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Comparative Assessment of Water Stress Responses in Sesame Reveals Contrasting Genotypes Differing in Xyloglucan Endotransglucosylase/Hydrolase

  • 期刊论文

  • 发表日期：

    2026年6月29日

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  • S. Sreepriya,
  • T. Girija,
  • M. S. Parvathi

摘要

Abstract

Germination and subsequent growth stages of sesame is often affected by drought and waterlogging stresses, respectively. With an aim to identify genotypes possessing stage specific water stress responses, laboratory and pot culture experiments were envisaged. In laboratory condition, fifteen sesame genotypes were subjected to polyethylene glycol (PEG) induced osmotic stress. Percentage of normal seedlings, root length, shoot length and vigour index I (VI) were observed to be negatively affected under PEG induced osmotic stress, whereas the proportion of abnormal seedlings and dead seeds increased. The same set of genotypes were subjected to waterlogging stress for a period of 72 h at vegetative stage in a pot culture. Based on biochemical and morphological performances across both stresses, genotypes were grouped into different clusters with a target to identify the ones with seedling level osmotic stress tolerance and vegetative stage waterlogging stress tolerance. The group of tolerant genotypes possessed higher survival rate and recovery response. Reduction in shoot and root dry weights, and plant height were minimal in the tolerant ones compared to susceptible genotypes. They also demonstrated an increase in root number in contrast to susceptible ones. Reactive oxygen species (ROS) production was higher in susceptible genotypes in terms of increased malondialdehyde (MDA) content while the catalase enzyme activity was lesser in contrast to the tolerant genotypes. Z scatter analysis of important morphological parameters under waterlogging and osmotic stress conditions revealed that the wild genotype Sesamum malabaricum possessed tolerance to both stresses, whereas CO 1 was susceptible to both stresses. Sequencing of XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE gene in these contrasting genotypes revealed sequence dissimilarity. In silico protein analysis revealed a change in domain constitution in the xyloglucan endotransglucosylase/hydrolase in the susceptible genotype compared to the tolerant one. Molecular docking studies revealed a reduction in cavity size of the enzyme in the susceptible genotype which plausibly affects its functionality.