Scholarlink

Abstract <p>This study examines the chloroplast genome (cpDNA) of <i>Senna alexandrina</i> to elucidate its nucleotide architecture and genic composition, providing new perspectives on its phylogenetic placement within the genus. The cpDNA structure was determined to span 159 753 bp and exhibits a quadripartite structural organization, comprising an 88 368 bp large single-copy (LSC) segment, an 18 721 bp small single-copy (SSC) segment, and two inverted repeat (IR) regions each spanning 26 241 bp. Annotation identified 131 functional genes, including 87 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. This configuration aligns with typical angiosperm plastome architecture, characterized by conserved LSC/IR/SSC partitioning and functional gene distribution patterns observed across plant lineages. Comparative genomic analyses with two phylogenetically proximate species revealed distinct features of <i>Senna alexandrina</i>, particularly in structural organization, nucleotide bias, codon preferences, and microsatellite distribution. A notable divergence was the lack of the <i>ycf3</i> and <i>ycf4</i> genes in its cpDNA a finding absent in previously studied <i>Senna</i> species. This study represents the inaugural detailed characterization of <i>Senna alexandrina</i>’s chloroplast genome, offering critical data for re-evaluating taxonomic boundaries between <i>Senna</i> and <i>Cassia</i>, which appear inconsistent with earlier classifications. The complete cpDNA sequence has been archived in GenBank (Accession: PP091277), serving as a foundational resource for future evolutionary and biotechnological studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative Analysis of the Complete Chloroplast Genome of Senna alexandrina: Molecular Features and Phylogenetic Relationships with Closely Related Species

  • 期刊论文

  • 发表日期：

    2026年3月18日

查看全文
  • L. Z. Aljumaiah

摘要

Abstract

This study examines the chloroplast genome (cpDNA) of Senna alexandrina to elucidate its nucleotide architecture and genic composition, providing new perspectives on its phylogenetic placement within the genus. The cpDNA structure was determined to span 159 753 bp and exhibits a quadripartite structural organization, comprising an 88 368 bp large single-copy (LSC) segment, an 18 721 bp small single-copy (SSC) segment, and two inverted repeat (IR) regions each spanning 26 241 bp. Annotation identified 131 functional genes, including 87 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. This configuration aligns with typical angiosperm plastome architecture, characterized by conserved LSC/IR/SSC partitioning and functional gene distribution patterns observed across plant lineages. Comparative genomic analyses with two phylogenetically proximate species revealed distinct features of Senna alexandrina, particularly in structural organization, nucleotide bias, codon preferences, and microsatellite distribution. A notable divergence was the lack of the ycf3 and ycf4 genes in its cpDNA a finding absent in previously studied Senna species. This study represents the inaugural detailed characterization of Senna alexandrina’s chloroplast genome, offering critical data for re-evaluating taxonomic boundaries between Senna and Cassia, which appear inconsistent with earlier classifications. The complete cpDNA sequence has been archived in GenBank (Accession: PP091277), serving as a foundational resource for future evolutionary and biotechnological studies.