Genome-Wide Identification and Expression Characteristic Analysis of the GRF Gene Family in Broussonetia papyrifera
摘要
Growth-regulating factors (GRFs) are plant-specific transcription factors. GRFs play pivotal roles in plant growth, development, and stress adaptation. In this study, a genome-wide analysis identified nine GRF family members in Broussonetia papyrifera (L.) L'Hér. ex Vent. (BpGRFs). Bioinformatics characterization revealed their physicochemical properties, gene structures, conserved domains, and evolutionary relationships. Phylogenetic analysis classified the BpGRFs into distinct clades, showing high homology with other dicot species. Expression profiling demonstrated that BpGRFs exhibit clear tissue specificity, with predominant expression in axillary buds and leaves, suggesting roles in rapid growth regulation. Under PEG-induced drought stress, the BpGRFs displayed time-specific expression patterns, with several members significantly upregulated at key stress time points. These findings indicate that BpGRFs may function cooperatively in mediating both growth and drought stress responses. This study provides important insights into the molecular functions of BpGRFs and offers potential targets for molecular breeding aimed at improving stress resilience and growth traits in B. papyrifera.