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Abstract <p>Seed germination marks the transition of dormancy to active plant growth. Accumulation of reactive oxygen species (ROS) is critical for seed coat rupture to facilitate the radicle emergence. The seed priming with nanoparticles (NPs) can be a promising avenue to improve seedling vigor, stress mitigation, and overall crop yield. Previous studies have shown that MgO-NPs exposure for more than 16 h can influence plant growth and development. However, there is a limited literature describing the role of NPs during the germination process. Therefore, in the present study the effect of short-term MgO-NPs priming on radish (<i>Raphanus sativus</i> (L.) Domin) seed germination was evaluated. Radish seeds were primed for 2 h with four different MgO-NPs concentrations, 10, 150, 250, and 500 µg/mL, before transferring to growth chambers for germination. Seeds were collected intermittently during germination, and results showed that 2 h MgO-NPs nanopriming enhanced germination than the untreated control. The order of germination time was observed as: 8 hap (h&#xa0;after priming) N-500 &gt; 9 hap N-250 &gt; 9.5 hap N-150 = 9.5 hap N10 &gt; 10 hap control. Biochemical analysis revealed that 500 µg/mL MgO-NPs induced a significant ROS burst at 8 hap with simultaneous reduction in the antioxidant potential. The ROS upsurge facilitated seed coat rupture and radicle emergence. In addition, MgO-NPs enhanced α-amylase activity, leading to higher carbohydrate and protein content to fuel seed germination. Increased salicylic acid levels and reduced auxin content further supported accelerated seed germination in MgO-NPs nanorpimed radish seed than the control. In conclusion, the present study demonstrates that time-bound nanopriming with MgO-NPs can influence germination via ROS singaling and regulation of key biochemical events. Further research on exposure durations beyond 2 h may help in identification of an effective MgO-NPs concentration for improved growth and yield in radish. Moreover, extending such studies in other plant species could establish MgO-NPs as sustainable growth-promoting alternative agriculture.</p>

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ROS Burst and Enhanced α-Amylase Activity Promoted Germination in Radish Seeds Nanoprimed with MgO-NPs

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • S. Chopra,
  • V. Kumar,
  • P. Guleria

摘要

Abstract

Seed germination marks the transition of dormancy to active plant growth. Accumulation of reactive oxygen species (ROS) is critical for seed coat rupture to facilitate the radicle emergence. The seed priming with nanoparticles (NPs) can be a promising avenue to improve seedling vigor, stress mitigation, and overall crop yield. Previous studies have shown that MgO-NPs exposure for more than 16 h can influence plant growth and development. However, there is a limited literature describing the role of NPs during the germination process. Therefore, in the present study the effect of short-term MgO-NPs priming on radish (Raphanus sativus (L.) Domin) seed germination was evaluated. Radish seeds were primed for 2 h with four different MgO-NPs concentrations, 10, 150, 250, and 500 µg/mL, before transferring to growth chambers for germination. Seeds were collected intermittently during germination, and results showed that 2 h MgO-NPs nanopriming enhanced germination than the untreated control. The order of germination time was observed as: 8 hap (h after priming) N-500 > 9 hap N-250 > 9.5 hap N-150 = 9.5 hap N10 > 10 hap control. Biochemical analysis revealed that 500 µg/mL MgO-NPs induced a significant ROS burst at 8 hap with simultaneous reduction in the antioxidant potential. The ROS upsurge facilitated seed coat rupture and radicle emergence. In addition, MgO-NPs enhanced α-amylase activity, leading to higher carbohydrate and protein content to fuel seed germination. Increased salicylic acid levels and reduced auxin content further supported accelerated seed germination in MgO-NPs nanorpimed radish seed than the control. In conclusion, the present study demonstrates that time-bound nanopriming with MgO-NPs can influence germination via ROS singaling and regulation of key biochemical events. Further research on exposure durations beyond 2 h may help in identification of an effective MgO-NPs concentration for improved growth and yield in radish. Moreover, extending such studies in other plant species could establish MgO-NPs as sustainable growth-promoting alternative agriculture.