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Abstract <p>A one-step sol-gel synthesis of organosilica matrices based on silicon tetrapolyethylene glycolate (STP<sub>EG</sub>) and silicon glycerolates (SiGl) at about pH 7.0 was performed to immobilize cells of the green algae <i>Chlamydomonas reinhardtii</i>. Infrared (IR) and Raman spectroscopy confirmed the formation of Si–O–Si and Si–O–C bonds, and the type of polymer in these matrices was determined. For the first time, scanning electron microscopy showed the formation of biohybrid “cell-in-shell” structures for the wild-type strain <i>C.&#xa0;reinhardtii</i> 137c. At the same time, when using mutant <i>C. reinhardtii</i> CC-503 deprived of their cell wall, effective cells immobilization has not been observed, indicating the key role of the cell wall in the immobilization process. Comparative analysis of matrices based on traditional precursors—tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES), revealed their toxicity to algal cells, although systems containing polyols ensured the preservation of cell morphology, but required optimization to increase cell viability. PAM fluorometry revealed a significant decrease in the photosynthetic activity of the algae cells of both strains studied (<i>F</i><sub>v</sub>/<i>F</i><sub>m</sub> &lt; 0.07), which is associated with the degradation of photosystem II. However, immobilization in agarose gel confirms the fundamental possibility of forming biohybrid systems with high viability of <i>C. reinhardtii</i> cells.</p>

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Immobilization of the Green Microalgae Chlamydomonas reinhardtii Cells in Polyolsilane-Based Matrices Using Sol-Gel Synthesis

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • E. S. Filippova,
  • V. V. Terentyev,
  • A. N. Zvonarev,
  • S. V. Alferov,
  • D. G. Lavrova

摘要

Abstract

A one-step sol-gel synthesis of organosilica matrices based on silicon tetrapolyethylene glycolate (STPEG) and silicon glycerolates (SiGl) at about pH 7.0 was performed to immobilize cells of the green algae Chlamydomonas reinhardtii. Infrared (IR) and Raman spectroscopy confirmed the formation of Si–O–Si and Si–O–C bonds, and the type of polymer in these matrices was determined. For the first time, scanning electron microscopy showed the formation of biohybrid “cell-in-shell” structures for the wild-type strain C. reinhardtii 137c. At the same time, when using mutant C. reinhardtii CC-503 deprived of their cell wall, effective cells immobilization has not been observed, indicating the key role of the cell wall in the immobilization process. Comparative analysis of matrices based on traditional precursors—tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES), revealed their toxicity to algal cells, although systems containing polyols ensured the preservation of cell morphology, but required optimization to increase cell viability. PAM fluorometry revealed a significant decrease in the photosynthetic activity of the algae cells of both strains studied (Fv/Fm < 0.07), which is associated with the degradation of photosystem II. However, immobilization in agarose gel confirms the fundamental possibility of forming biohybrid systems with high viability of C. reinhardtii cells.