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Abstract <p>This article describes the negative effects of exogenously applied and endogenously produced jasmonates in the tolerance of bread wheat (<i>Triticum aestivum</i> L.) plants of the Saratovskaya-60 cultivar to excessive light conditions. While no significant effect of jasmonates on the variable chlorophyll fluorescence parameters is evident under moderate and low light conditions, the negative effect of jasmonates at high light intensities is evident on a “rapid light curve” of methyl jasmonate (MeJA)-treated plants and transgenic plants Tr-20 overexpressing <i>12-OXOPHYTODIENOATE REDUCTASE 3</i> (<i>AtOPR3</i>), a jasmonate biosynthesis gene from <i>Arabidopsis thaliana</i>. The analysis of fast chlorophyll fluorescence kinetics with high-time resolution (OJIP test) confirmed the difference between Tr-20, MeJA-treated and mock treated ‘Saratovskaya-60’. Two hours of plant leaves incubation under photoinhibitory light induce clearly visible changes in OJIP kinetics, and these changes are more pronounced in MeJA-treated and Tr-20 leaves as lower values of kinetic transient points J, I, and P. Subsequent two-hour incubation under low light leads to an almost complete recovery of the OJIP kinetics in leaves of mock treated ‘Saratovskaya-60’ leaves, while only partial recovery is observed in the leaves of MeJA-treated and Tr-20 plants. The transgenic line Tr-3, which under high light conditions does not accumulate more jasmonates than non-transgenic parental line, does not differ in tolerance to photoinhibition from the non-transgenic plants, confirming that the increased level of jasmonates is necessary to compromise the tolerance to photoinhibition. Analysis of stress hormones revealed the role of jasmonates in modulating the level of abscisic acid and salicylic acid during wheat leaves adaptation to high-light conditions. The data obtained lay the foundation for further studies of complex hormonal cross-talk in wheat leaves under light stress conditions.</p>

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The Negative Role of Jasmonic Acid in Wheat Tolerance to Excessive Light

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  • 发表日期：

    2025年12月16日

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  • A. A. Khorobrykh,
  • E. A. Degtyaryov,
  • A. V. Pigolev,
  • D. Miroshnichenko,
  • S. V. Dolgov,
  • T. V. Savchenko

摘要

Abstract

This article describes the negative effects of exogenously applied and endogenously produced jasmonates in the tolerance of bread wheat (Triticum aestivum L.) plants of the Saratovskaya-60 cultivar to excessive light conditions. While no significant effect of jasmonates on the variable chlorophyll fluorescence parameters is evident under moderate and low light conditions, the negative effect of jasmonates at high light intensities is evident on a “rapid light curve” of methyl jasmonate (MeJA)-treated plants and transgenic plants Tr-20 overexpressing 12-OXOPHYTODIENOATE REDUCTASE 3 (AtOPR3), a jasmonate biosynthesis gene from Arabidopsis thaliana. The analysis of fast chlorophyll fluorescence kinetics with high-time resolution (OJIP test) confirmed the difference between Tr-20, MeJA-treated and mock treated ‘Saratovskaya-60’. Two hours of plant leaves incubation under photoinhibitory light induce clearly visible changes in OJIP kinetics, and these changes are more pronounced in MeJA-treated and Tr-20 leaves as lower values of kinetic transient points J, I, and P. Subsequent two-hour incubation under low light leads to an almost complete recovery of the OJIP kinetics in leaves of mock treated ‘Saratovskaya-60’ leaves, while only partial recovery is observed in the leaves of MeJA-treated and Tr-20 plants. The transgenic line Tr-3, which under high light conditions does not accumulate more jasmonates than non-transgenic parental line, does not differ in tolerance to photoinhibition from the non-transgenic plants, confirming that the increased level of jasmonates is necessary to compromise the tolerance to photoinhibition. Analysis of stress hormones revealed the role of jasmonates in modulating the level of abscisic acid and salicylic acid during wheat leaves adaptation to high-light conditions. The data obtained lay the foundation for further studies of complex hormonal cross-talk in wheat leaves under light stress conditions.