Abstract <p>Accumulation of Zinc in soil at high concentration leads to drastic changes in the structure of microbial communities and a decrease in soil fertility. Legumes growing on Zn-contaminated soils are negatively affected. Based on our previous work, <i>Trigonella foenum-graecum</i> L. was cultivated on a chosen concentration of zinc (0.75 mM ZnSO₄) under three different regimes of nitrogen sources, without nitrogen, nitrogen fertilisation (with urea) or nitrogen symbiotic fixation (inoculated with specific rhizobia). Results showed a significant zinc effect on shoot dry weight with a reduction more pronounced in symbiotic regime (36.9%). Concerning roots, plants showed only a reduction of 12.6% in nitrogen fertilisation regime against 31% and 58.4% with zero nitrogen and symbiotic nitrogen fixation regime respectively. Moreover, number and dry weight of nodules were significantly affected. The supply of exogenous zinc (0.75 mM) induced a significant increase of this metal in shoot for plants without nitrogen (0.6 mg/g DW) and plants with nitrogen fertilisation (0.26 mg/g DW). The notable result was the increase of zinc content in root and nodules of plants inoculated with rhizobia under zinc supply. Zinc showed a significant increase of Ca content in shoots and roots in inoculated plants. In conclusion, the effect of zinc on <i>T. foenum-graecum</i> is variable according to the nitrogen source with superiority in growth parameters for nitrogen fertilisation and preference for symbiotic nitrogen fixation for phytostabilisation of zinc and translocation and uptake of nutrients.</p>