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Abstract <p>Cloning of the <i>SaSLAH3</i> gene (OQ271227.1), an <i>AtSLAH3</i> ortholog, member of the slow anion channels family (SLAC/SLAH), has been carried out from the euhalophyte <i>Suaeda altissima</i>. Under hydroponic conditions, these species related to Amaranthaceae is capable to complete life cycle on nutrient solutions containing up to 1 M NaCl. Cloning of <i>SaSLAH3</i> was performed based on a putative <i>SaSLAH3</i> similarity with <i>SLAH3</i> homologs from the related <i>Suaeda glauca</i> and <i>Suaeda fruticose</i> through assembled transcriptomes previously. Membrane topology, analyzed by <i>in silico</i> approach, the 3-D structure, presence of conservative phenylalanine-bearing motives and as well as SaSLAH3 MEME-generated sequence motif patterns, all of these features were found typical for the SLAC/SLAH channels and confirmed predicted SaSLAH3 is a new member of this family. Phylogenetic relationship analysis demonstrated the evolutionary SLAC-like proteins divergence into three distinct groups with location in SLAH2/3 clade. Expression profile investigation by quantitative real-time PCR revealed roots and leaves <i>SaSLAH3</i> transcript level increment upon NaCl elevation with sufficient nitrate supply and nitrate deficiency. Probably, SaSLAH3 plays an important role in chloride loading enhancing into root xylem and, thereafter in chloride delivery to shoot under salinity. Another SaSLAH3 physiological role may be cytoplasm nitrate concentration regulation to ensure proper <i>S. altissima</i> nitrogen nutrition to strong competition between nitrate and chloride ions.</p>

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Molecular Cloning of SaSLAH3, a Novel Slow Anion Channel SLAC1/SLAH Family Gene, from Euhalophyte Suaeda altissima (L.) Pall. and Analysis of the Predicted Protein

  • 期刊论文

  • 发表日期：

    2025年7月5日

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  • E. I. Rostovtseva,
  • D. E. Khramov,
  • O. V. Lobreva,
  • L. A. Khalilova,
  • I. G. Tarakanov,
  • I. V. Karpichev,
  • Y. V. Balnokin

摘要

Abstract

Cloning of the SaSLAH3 gene (OQ271227.1), an AtSLAH3 ortholog, member of the slow anion channels family (SLAC/SLAH), has been carried out from the euhalophyte Suaeda altissima. Under hydroponic conditions, these species related to Amaranthaceae is capable to complete life cycle on nutrient solutions containing up to 1 M NaCl. Cloning of SaSLAH3 was performed based on a putative SaSLAH3 similarity with SLAH3 homologs from the related Suaeda glauca and Suaeda fruticose through assembled transcriptomes previously. Membrane topology, analyzed by in silico approach, the 3-D structure, presence of conservative phenylalanine-bearing motives and as well as SaSLAH3 MEME-generated sequence motif patterns, all of these features were found typical for the SLAC/SLAH channels and confirmed predicted SaSLAH3 is a new member of this family. Phylogenetic relationship analysis demonstrated the evolutionary SLAC-like proteins divergence into three distinct groups with location in SLAH2/3 clade. Expression profile investigation by quantitative real-time PCR revealed roots and leaves SaSLAH3 transcript level increment upon NaCl elevation with sufficient nitrate supply and nitrate deficiency. Probably, SaSLAH3 plays an important role in chloride loading enhancing into root xylem and, thereafter in chloride delivery to shoot under salinity. Another SaSLAH3 physiological role may be cytoplasm nitrate concentration regulation to ensure proper S. altissima nitrogen nutrition to strong competition between nitrate and chloride ions.