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Abstract <p>The basic helix-loop-helix (bHLH) transcription factors (TFs) are one of the largest families of TFs in plants, which can form a relatively stable and interacting complex regulatory network and play major roles in plant growth and development, as well as regulating plant responses to abiotic stress. As a member of the bHLH family of TFs, MUTE has been little studied in terms of its role in response to abiotic stresses. In this study, the <i>CsMUTE</i> gene was isolated from cucumber (<i>Cucumis sativus</i> L.) leaves, and its structure and function were preliminarily analyzed. The coding sequence (CDS) of the CsMUTE consists of 573 bp, which encodes 190 amino acids. The CsMUTE contains a typical bHLH structural domain. Cucumber composite plants that overexpressed <i>CsMUTE</i> in roots were obtained by <i>Agrobacterium rhizogenes</i> K599-mediated hairy root transformation. The overexpression composite plants exhibited higher salt tolerance than the empty vector control composite plants. The heterologous overexpression of <i>CsMUTE</i> in Arabidopsis further confirmed the salt tolerance function of <i>CsMUTE</i>. These results provide a new perspective for a deep understanding of biological functions and regulatory mechanisms of bHLH family members in plants.</p>

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Characterization of the CsMUTE Gene from Cucumis sativus and Its Function in Response to Salt Stress

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • S. Chen,
  • C. X. Du,
  • M. X. Lai,
  • M. Chen,
  • H. F. Fan

摘要

Abstract

The basic helix-loop-helix (bHLH) transcription factors (TFs) are one of the largest families of TFs in plants, which can form a relatively stable and interacting complex regulatory network and play major roles in plant growth and development, as well as regulating plant responses to abiotic stress. As a member of the bHLH family of TFs, MUTE has been little studied in terms of its role in response to abiotic stresses. In this study, the CsMUTE gene was isolated from cucumber (Cucumis sativus L.) leaves, and its structure and function were preliminarily analyzed. The coding sequence (CDS) of the CsMUTE consists of 573 bp, which encodes 190 amino acids. The CsMUTE contains a typical bHLH structural domain. Cucumber composite plants that overexpressed CsMUTE in roots were obtained by Agrobacterium rhizogenes K599-mediated hairy root transformation. The overexpression composite plants exhibited higher salt tolerance than the empty vector control composite plants. The heterologous overexpression of CsMUTE in Arabidopsis further confirmed the salt tolerance function of CsMUTE. These results provide a new perspective for a deep understanding of biological functions and regulatory mechanisms of bHLH family members in plants.