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Abstract <p><i>Delay of Germination 1</i> (<i>DOG1</i>) is associated with dormancy, which has a critical role in the dormancy process of various species. However, the mechanism of <i>DOG1</i> in peach dormancy remains unclear. To examine the role of <i>PpDOG1</i> in peach (<i>Prunus persica</i> (L.) Batsch) bud dormancy, we harvested the annual branches of the ‘Yingshuanghong’ peach. We performed bioinformatics analysis and fluorescence quantitative analysis, generating a transgenic tobacco line overexpressing <i>PpDOG1</i>. The dormancy process of peach buds can be divided into three stages: endodormancy, dormancy transformation, and ecodormancy. The germination rate of peach buds was closely related to low temperatures. This study revealed that the change in ABA (abscisic acid) level during peach dormancy was closely associated with the dormancy state. The expression of the <i>PpDOG1</i> gene was high in the dormancy transition stage but gradually decreased during the ecological dormancy stage, suggesting that <i>PpDOG1</i> might positively regulate the dormancy of peach buds. Further experiments demonstrated that the expression of <i>PpDOG1</i> increased significantly after treatment with ABA and decreased after treatment with its inhibitors, suggesting that it may impact dormancy by modulating ABA levels. Moreover, transgenic tobacco seeds overexpressing <i>PpDOG1</i> had a slower germination rate, supporting the potential of the <i>PpDOG1</i> gene as a positive regulator of peach dormancy.</p>

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Molecular Mechanism of the PpDOG1 Gene Regulating Bud-Break in Peach

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • T. He,
  • S. Li,
  • G. Tian,
  • W. Zhao,
  • Q. Wu,
  • W. Xiao,
  • L. Li

摘要

Abstract

Delay of Germination 1 (DOG1) is associated with dormancy, which has a critical role in the dormancy process of various species. However, the mechanism of DOG1 in peach dormancy remains unclear. To examine the role of PpDOG1 in peach (Prunus persica (L.) Batsch) bud dormancy, we harvested the annual branches of the ‘Yingshuanghong’ peach. We performed bioinformatics analysis and fluorescence quantitative analysis, generating a transgenic tobacco line overexpressing PpDOG1. The dormancy process of peach buds can be divided into three stages: endodormancy, dormancy transformation, and ecodormancy. The germination rate of peach buds was closely related to low temperatures. This study revealed that the change in ABA (abscisic acid) level during peach dormancy was closely associated with the dormancy state. The expression of the PpDOG1 gene was high in the dormancy transition stage but gradually decreased during the ecological dormancy stage, suggesting that PpDOG1 might positively regulate the dormancy of peach buds. Further experiments demonstrated that the expression of PpDOG1 increased significantly after treatment with ABA and decreased after treatment with its inhibitors, suggesting that it may impact dormancy by modulating ABA levels. Moreover, transgenic tobacco seeds overexpressing PpDOG1 had a slower germination rate, supporting the potential of the PpDOG1 gene as a positive regulator of peach dormancy.