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Abstract <p>The role of the enzymes succinate dehydrogenase (SDH) and succinate-semialdehyde dehydrogenase (SSADH) in the regulation of its metabolism in wheat leaves (<i>Triticum aestivum</i> L.) under salt stress conditions and the expression levels of genes encoding these enzymes are discussed. At the level of SSADH, it was found that the maximum enzymatic activity was recorded in the first 6 h and was accompanied by an increase in <i>TaSSADH1</i> gene expression. Comparative analysis of these results with those previously obtained on maize leaves confirms that the induction of the gamma-aminobutyric acid shunt (GABA shunt) is similar in plants with C3- and C4-type metabolism and may be independent of their tolerance to salinity. Increasing the rate of functioning of SDH and oxidation of succinate in the mitochondria of wheat leaves was gradual over the 24 h of the experiment, unlike corn, which is due to the different resistance to salt stress of these plants. Since wheat is more resistant to salt stress, the activation of SDH function occurs faster than in corn. It has been established that <i>TaSDH1, TaSDH2, TaSDH3-2, TaSDH4</i>, and <i>TaSSADH1</i> gene expression in wheat leaves under salinity can be regulated by specific transcription factors, such as WRKY and NAC, the sites of which are found in the promoter regions of these genes.</p>

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Functioning of Succinate Metabolism Enzymes in Wheat Leaves under Salt Stress

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • A. T. Eprintsev,
  • D. N. Fedorin,
  • O. J. Flores Caro

摘要

Abstract

The role of the enzymes succinate dehydrogenase (SDH) and succinate-semialdehyde dehydrogenase (SSADH) in the regulation of its metabolism in wheat leaves (Triticum aestivum L.) under salt stress conditions and the expression levels of genes encoding these enzymes are discussed. At the level of SSADH, it was found that the maximum enzymatic activity was recorded in the first 6 h and was accompanied by an increase in TaSSADH1 gene expression. Comparative analysis of these results with those previously obtained on maize leaves confirms that the induction of the gamma-aminobutyric acid shunt (GABA shunt) is similar in plants with C3- and C4-type metabolism and may be independent of their tolerance to salinity. Increasing the rate of functioning of SDH and oxidation of succinate in the mitochondria of wheat leaves was gradual over the 24 h of the experiment, unlike corn, which is due to the different resistance to salt stress of these plants. Since wheat is more resistant to salt stress, the activation of SDH function occurs faster than in corn. It has been established that TaSDH1, TaSDH2, TaSDH3-2, TaSDH4, and TaSSADH1 gene expression in wheat leaves under salinity can be regulated by specific transcription factors, such as WRKY and NAC, the sites of which are found in the promoter regions of these genes.