<b>Abstract</b>— <p>In ten relevant <i>Staphylococcus aureus</i> targets, multiple docking of 254 known antimicrobial quinazolinone derivatives and 71 new quinazolinone derivatives was performed. Using a new original architecture of a modular multi-target fully connected convolutional neural network based on the correlation convolution of multiple docking energy spectra into relevant targets, a model of anti-<i>S. aureus</i> activity of chemical compounds has been constructed. The threshold value of the total energy of this neural network, separating the high-affinity and low-affinity compounds, has been determined as <i>V</i><sub>0</sub> &gt; 266.5. The limit value of anti-<i>S. aureus</i> activity, the minimum inhibitory concentration, which separated fairly active and low-active compounds, was determined as MIC &lt; 112.5 μg/mL. Using the obtained model, the accuracy of the prediction on the training set for anti-<i>S. aureus</i> activity of quinazolinone derivatives was assessed (<i>Acc</i> = 78.9%), which corresponded to the significance level <i>p</i> = 3.44 × 10<sup>–12</sup>. Prognosis of the anti-<i>S. aureus</i> activity of 71&#xa0;new quinazolinone derivatives was carried out, with the forecast efficiency of <i>PreHigh</i> = 73.2%. A substance with anti-<i>S. aureus</i> activity experimentally confirmed in vitro was found. The developed neural network technology can be recommended as a new method of artificial intelligence for <i>in silico</i> searching the antimicrobial substances.</p>

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In Silico Search for Antimicrobial Compounds Using Multi-Target Modular Fully Connected Convolutional Neural Network Based on Multiple Docking

  • P. M. Vassiliev,
  • A. V. Golubeva,
  • I. S. Stepanenko,
  • V. A. Kosov,
  • A. A. Ozerov,
  • O. V. Kuzminov,
  • M. A. Perfilev,
  • A. N. Kochetkov

摘要

Abstract

In ten relevant Staphylococcus aureus targets, multiple docking of 254 known antimicrobial quinazolinone derivatives and 71 new quinazolinone derivatives was performed. Using a new original architecture of a modular multi-target fully connected convolutional neural network based on the correlation convolution of multiple docking energy spectra into relevant targets, a model of anti-S. aureus activity of chemical compounds has been constructed. The threshold value of the total energy of this neural network, separating the high-affinity and low-affinity compounds, has been determined as V0 > 266.5. The limit value of anti-S. aureus activity, the minimum inhibitory concentration, which separated fairly active and low-active compounds, was determined as MIC < 112.5 μg/mL. Using the obtained model, the accuracy of the prediction on the training set for anti-S. aureus activity of quinazolinone derivatives was assessed (Acc = 78.9%), which corresponded to the significance level p = 3.44 × 10–12. Prognosis of the anti-S. aureus activity of 71 new quinazolinone derivatives was carried out, with the forecast efficiency of PreHigh = 73.2%. A substance with anti-S. aureus activity experimentally confirmed in vitro was found. The developed neural network technology can be recommended as a new method of artificial intelligence for in silico searching the antimicrobial substances.