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Abstract <p>The acidobacterial genus <i>Paludibaculum</i> accommodates a single species, <i>Paludibaculum fermentans</i>, which was described one decade ago based on characterization of a single isolate, strain P105<sup>T</sup>. This isolate was obtained from the littoral wetland of a boreal lake located on Valaam Island, Karelia, Northern Russia. Members of this species are facultatively anaerobic and mildly acidophilic bacteria that inhabit wetlands and groundwater bodies and belong to the family <i>Bryobacteraceae</i>, order <i>Bryobacterales</i> of the class <i>Terriglobia</i>. The specific feature of <i>Paludibaculum fermentans</i> is its ability to couple Fe(III) reduction to the fermentation of sugars and some polysaccharides under anoxic conditions. The ability to degrade cellulose, however, has not been reported for these acidobacteria. In this study, we characterize a new member of the genus <i>Paludibaculum</i>, strain T20B24, which was isolated from a boreal fen using enrichment culture emended with xylan. The 16S rRNA gene sequence similarity between the strains T20B24 and P105<sup>T</sup> was 99.85%. Cell morphology of strain T20B24 was distinct from that in P105<sup>T</sup>, and the two isolates displayed slight differences with regard to pH optima and substrate utilization patterns. Notably, strain T20B24 was capable of using fibrous cellulose as growth substrate. The genome sequence of this acidobacterium determined by using a combination of Illumina and Nanopore technologies was 10.1 Mb in size and contained 8096 protein-coding genes. An extremely wide array of CAZymes encoded in the genomes of strain T20B24 and <i>P. fermentans</i> P105<sup>T</sup> included several potential cellulases from GH family 5 and one potential xylanase from GH family 8; the phylogeny of these proteins was analyzed. The proteins from subfamily GH5_25 fell within the large clade of putative acidobacterial cellulases encoded in metagenomes from soils and peatlands. Taken together, these results confirm the presence of cellulose-degrading capabilities in acidobacteria of the genus <i>Paludibaculum</i>.</p>

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Cellulolytic Capabilities in Paludibaculum sp. T20B24, a Novel Acidobacterium from a Boreal Fen

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  • 发表日期：

    2025年11月17日

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  • I. S. Kulichevskaya,
  • A. A. Ivanova,
  • D. G. Naumoff,
  • S. N. Dedysh

摘要

Abstract

The acidobacterial genus Paludibaculum accommodates a single species, Paludibaculum fermentans, which was described one decade ago based on characterization of a single isolate, strain P105T. This isolate was obtained from the littoral wetland of a boreal lake located on Valaam Island, Karelia, Northern Russia. Members of this species are facultatively anaerobic and mildly acidophilic bacteria that inhabit wetlands and groundwater bodies and belong to the family Bryobacteraceae, order Bryobacterales of the class Terriglobia. The specific feature of Paludibaculum fermentans is its ability to couple Fe(III) reduction to the fermentation of sugars and some polysaccharides under anoxic conditions. The ability to degrade cellulose, however, has not been reported for these acidobacteria. In this study, we characterize a new member of the genus Paludibaculum, strain T20B24, which was isolated from a boreal fen using enrichment culture emended with xylan. The 16S rRNA gene sequence similarity between the strains T20B24 and P105T was 99.85%. Cell morphology of strain T20B24 was distinct from that in P105T, and the two isolates displayed slight differences with regard to pH optima and substrate utilization patterns. Notably, strain T20B24 was capable of using fibrous cellulose as growth substrate. The genome sequence of this acidobacterium determined by using a combination of Illumina and Nanopore technologies was 10.1 Mb in size and contained 8096 protein-coding genes. An extremely wide array of CAZymes encoded in the genomes of strain T20B24 and P. fermentans P105T included several potential cellulases from GH family 5 and one potential xylanase from GH family 8; the phylogeny of these proteins was analyzed. The proteins from subfamily GH5_25 fell within the large clade of putative acidobacterial cellulases encoded in metagenomes from soils and peatlands. Taken together, these results confirm the presence of cellulose-degrading capabilities in acidobacteria of the genus Paludibaculum.