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Abstract <p>The composition of four stable halophilic methylotrophic bacterial communities (3Sol, GBL, SBL, 8sh_L) formed in laboratory conditions as a result of long-term periodic cultivation on a mineral medium with methanol of water samples from salt lakes Dunino (Orenburg oblast), Bolshoe Medvezhye, and Maloe Medvezhye (Kurgan oblast), as well as the Indian Ocean, was studied by the 16S rRNA gene metabarcoding method. Bioinformatics analysis was used to obtain 35 892 reads, which were grouped into 105 amplified sequence variants (ASVs). It was shown that representatives of the family <i>Methylophagaceae</i>, methylotrophs of the genus <i>Methylophaga</i> prevailed in all four halophilic methylotrophic communities, and representatives of the family <i>Halomonadaceae</i>, bacteria of the genera <i>Vreelandella</i> and <i>Halomonas</i> prevailed among the associated halophilic heterotrophic microorganisms. Methylotrophic strains of the studied communities were represented by uncultured bacteria, probably metabolically associated with heterotrophs. The strain <i>Vreelandella titanicae</i> was isolated from the 3Sol methylotrophic community and studied in an artificial association with the methylotroph <i>Methylophaga marina</i> VKM B-2056<sup>T</sup>.</p>

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Molecular Analysis of Phylogenetic Diversity of Halophilic Methylotrophic Bacterial Communities

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • M. N. Shustova,
  • Yu. N. Kocharovskaya,
  • Ya. A. Delegan,
  • E. N. Kaparullina,
  • N. V. Doronina

摘要

Abstract

The composition of four stable halophilic methylotrophic bacterial communities (3Sol, GBL, SBL, 8sh_L) formed in laboratory conditions as a result of long-term periodic cultivation on a mineral medium with methanol of water samples from salt lakes Dunino (Orenburg oblast), Bolshoe Medvezhye, and Maloe Medvezhye (Kurgan oblast), as well as the Indian Ocean, was studied by the 16S rRNA gene metabarcoding method. Bioinformatics analysis was used to obtain 35 892 reads, which were grouped into 105 amplified sequence variants (ASVs). It was shown that representatives of the family Methylophagaceae, methylotrophs of the genus Methylophaga prevailed in all four halophilic methylotrophic communities, and representatives of the family Halomonadaceae, bacteria of the genera Vreelandella and Halomonas prevailed among the associated halophilic heterotrophic microorganisms. Methylotrophic strains of the studied communities were represented by uncultured bacteria, probably metabolically associated with heterotrophs. The strain Vreelandella titanicae was isolated from the 3Sol methylotrophic community and studied in an artificial association with the methylotroph Methylophaga marina VKM B-2056T.