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Abstract <p>This investigation explored the impact of co-cultivation on the phospholipidome of two xylotrophic basidiomycetes. As a model, <i>Flammulina velutipes</i> and <i>Sparassis crispa</i> were grown as surface monocultures and paired in co-culture, and molecular species of phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were quantified by targeted LC-ESI-QqQ-MS/MS. Under co-cultivation, <i>F. velutipes</i> exhibited slower growth with altered colony morphology and decreases in PC, PE, and phosphatidic acid, together with a shift in the PC profile (decreased 18:1_18:2; increased 18:2_18:3; loss of molecular species with C20- and C22 very-long-chain fatty acids). These changes occurred alongside down-regulation of Kennedy-pathway genes and increased expression of the PE-methylation route. In <i>S. crispa</i>, biomass increased and PC, PE rose via higher 18:1_18:2 and 18:2_18:2, with up-regulation of Kennedy-pathway genes. Overall, co-cultivation with a specific antagonist can initiate PC biosynthesis via PE methylation—a potential tolerance mechanism that enables xylotrophic basidiomycetes to develop under competitive conditions.</p>

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Effect of Co-Cultivation of Xylotrophic Fungi Flammulina velutipes and Sparassis crispa (Basidiomycota) on the Molecular Profile and Metabolism of Phospholipids

  • 期刊论文

  • 发表日期：

    2026年2月26日

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  • B. S. Manzhieva,
  • S. V. Senik,
  • L. G. Maloshenok,
  • A. A. Kiyashko,
  • S. A. Bruskin,
  • E. B. Serebryakov,
  • A. A. Khakulova,
  • E. R. Kotlova

摘要

Abstract

This investigation explored the impact of co-cultivation on the phospholipidome of two xylotrophic basidiomycetes. As a model, Flammulina velutipes and Sparassis crispa were grown as surface monocultures and paired in co-culture, and molecular species of phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were quantified by targeted LC-ESI-QqQ-MS/MS. Under co-cultivation, F. velutipes exhibited slower growth with altered colony morphology and decreases in PC, PE, and phosphatidic acid, together with a shift in the PC profile (decreased 18:1_18:2; increased 18:2_18:3; loss of molecular species with C20- and C22 very-long-chain fatty acids). These changes occurred alongside down-regulation of Kennedy-pathway genes and increased expression of the PE-methylation route. In S. crispa, biomass increased and PC, PE rose via higher 18:1_18:2 and 18:2_18:2, with up-regulation of Kennedy-pathway genes. Overall, co-cultivation with a specific antagonist can initiate PC biosynthesis via PE methylation—a potential tolerance mechanism that enables xylotrophic basidiomycetes to develop under competitive conditions.