Scholarlink

Abstract <p>Halotolerant bacteria of the genera <i>Bacillus, Microbacterium, Glutamicibacter, Brevibacterium, Rhodococcus, Pseudomonas,</i> and <i>Thalassospira,</i> as well as a halophilic bacterium of the genus <i>Halomonas</i>, previously isolated from saline ecotopes of the northern region of Perm Krai, were studied for their ability to grow at low temperatures. It was found that strains of bacteria of the genera <i>Rhodococcus, Glutamicibacter, Microbacterium, Pseudomonas, Brevibacterium, Thalassospira</i>, and <i>Halomonas</i> grew at a temperature of 5°C. Bacteria of the genera <i>Brevibacterium</i> and <i>Rhodococcus</i> grew at 0°C. Cryoprotective solutes were studied using proton magnetic resonance spectroscopy. Since in natural habitats bacterial cells may attach to the surfaces of solid particles and form microcolonies, cryoprotectors were studied by growing bacterial strains on the surface of an agar medium. Using the psychrotrophic culture of <i>Rhodococcus</i> sp. SMB38 as an example, the dependence of the osmolyte composition on cultivation conditions was studied. When grown on an agar medium without sodium chloride at 25°C, the cells of <i>Rhodococcus</i> sp. strain SMB38 synthesized ectoine, hydroxyectoine, trehalose, and alanine. The proportion of trehalose (48%) was higher compared to other compounds. With a decrease in temperature, the intracellular amount of trehalose, ectoine, and alanine increased, with the proportion of trehalose was higher compared to other compounds (55%). The same patterns were noted when growing the strain on a solid medium containing 2% NaCl. However, the ratio of compounds changed under cold conditions: the largest proportion of 48% was that of ectoine. In this regard, cryoprotective substances were studied by culturing cold-resistant strains on a solid medium with 2% NaCl. It was shown that the cells of bacteria of the genera <i>Halomonas</i> and <i>Thalassospira</i> accumulated ectoine. In the cells of bacteria of the genera <i>Glutamicibacter</i> and <i>Pseudomonas</i>, trehalose performed the function of a cryoprotector. The psychrotrophic culture of the genus <i>Brevibacterium</i> accumulated trehalose, ectoine, and alanine in the cells. This is the first report on an increase in the amount of alanine in bacterial cells with a decrease in temperature, which may indicate involvement of this amino acid in adaptation to cold.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Adaptation to Growth at Low Temperature of Psychrotrophic Bacteria Isolated from Soil of the Northern Perm Krai

  • 期刊论文

  • 发表日期：

    2025年6月14日

查看全文
  • L. N. Anan’ina,
  • A. A. Gorbunov,
  • V. S. Aleev,
  • O. V. Yastrebova

摘要

Abstract

Halotolerant bacteria of the genera Bacillus, Microbacterium, Glutamicibacter, Brevibacterium, Rhodococcus, Pseudomonas, and Thalassospira, as well as a halophilic bacterium of the genus Halomonas, previously isolated from saline ecotopes of the northern region of Perm Krai, were studied for their ability to grow at low temperatures. It was found that strains of bacteria of the genera Rhodococcus, Glutamicibacter, Microbacterium, Pseudomonas, Brevibacterium, Thalassospira, and Halomonas grew at a temperature of 5°C. Bacteria of the genera Brevibacterium and Rhodococcus grew at 0°C. Cryoprotective solutes were studied using proton magnetic resonance spectroscopy. Since in natural habitats bacterial cells may attach to the surfaces of solid particles and form microcolonies, cryoprotectors were studied by growing bacterial strains on the surface of an agar medium. Using the psychrotrophic culture of Rhodococcus sp. SMB38 as an example, the dependence of the osmolyte composition on cultivation conditions was studied. When grown on an agar medium without sodium chloride at 25°C, the cells of Rhodococcus sp. strain SMB38 synthesized ectoine, hydroxyectoine, trehalose, and alanine. The proportion of trehalose (48%) was higher compared to other compounds. With a decrease in temperature, the intracellular amount of trehalose, ectoine, and alanine increased, with the proportion of trehalose was higher compared to other compounds (55%). The same patterns were noted when growing the strain on a solid medium containing 2% NaCl. However, the ratio of compounds changed under cold conditions: the largest proportion of 48% was that of ectoine. In this regard, cryoprotective substances were studied by culturing cold-resistant strains on a solid medium with 2% NaCl. It was shown that the cells of bacteria of the genera Halomonas and Thalassospira accumulated ectoine. In the cells of bacteria of the genera Glutamicibacter and Pseudomonas, trehalose performed the function of a cryoprotector. The psychrotrophic culture of the genus Brevibacterium accumulated trehalose, ectoine, and alanine in the cells. This is the first report on an increase in the amount of alanine in bacterial cells with a decrease in temperature, which may indicate involvement of this amino acid in adaptation to cold.