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Abstract <p>Soil ecosystems consist of diverse microbial communities with great potential for ecological and biotechnological applications. These communities encounter various abiotic stresses, which activate the transient overexpression of heat shock proteins (HSPs). In the present study, a soil bacterium was isolated and identified (biochemical characterization and 16S rRNA gene sequencing) as <i>Bacillus haynesii</i> strain PSK.N4, and its growth parameters were optimized before exposure to heat, salt, pH, and antibiotic stress conditions. The studied abiotic and antibiotic stress was induced by subjecting the bacterium to sublethal growth conditions. Comparative protein expression under stress conditions was analyzed using SDS-PAGE, protein stabilization via protein aggregation assays, and survival (colony counts). Short-term (10 min) pretreatment with the stressors showed elevated overall tolerance of bacterium to lethal conditions, as evidenced by moderately enhanced total content of soluble intracellular protein, better protein stabilization, comparatively over-expressed stress response proteins, and relatively enhanced cell survival. These findings revealed that cells grown under optimal conditions were more susceptible to lethal environments than stressed cells, with their enhanced tolerance linked to the overexpression of twenty distinct stress response proteins of ~17–88 kDa. These insights offer the potential for developing strategies to improve microbial resilience for various applications, including bacterial bioprocessing, bioremediation, and infectious disease management.</p>

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Stress Response Proteins Are the Reliant Factors for Resilience in the Soil Bacterium Bacillus haynesii PSK.N4

  • 期刊论文

  • 发表日期：

    2025年7月15日

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  • N. P. Dalbanjan,
  • A. J. Kadapure,
  • S. K. Praveen Kumar

摘要

Abstract

Soil ecosystems consist of diverse microbial communities with great potential for ecological and biotechnological applications. These communities encounter various abiotic stresses, which activate the transient overexpression of heat shock proteins (HSPs). In the present study, a soil bacterium was isolated and identified (biochemical characterization and 16S rRNA gene sequencing) as Bacillus haynesii strain PSK.N4, and its growth parameters were optimized before exposure to heat, salt, pH, and antibiotic stress conditions. The studied abiotic and antibiotic stress was induced by subjecting the bacterium to sublethal growth conditions. Comparative protein expression under stress conditions was analyzed using SDS-PAGE, protein stabilization via protein aggregation assays, and survival (colony counts). Short-term (10 min) pretreatment with the stressors showed elevated overall tolerance of bacterium to lethal conditions, as evidenced by moderately enhanced total content of soluble intracellular protein, better protein stabilization, comparatively over-expressed stress response proteins, and relatively enhanced cell survival. These findings revealed that cells grown under optimal conditions were more susceptible to lethal environments than stressed cells, with their enhanced tolerance linked to the overexpression of twenty distinct stress response proteins of ~17–88 kDa. These insights offer the potential for developing strategies to improve microbial resilience for various applications, including bacterial bioprocessing, bioremediation, and infectious disease management.