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Abstract <p>This is the first report on mapping the RNA-DNA hybrids in a prokaryotic genome. Using the S9.6 antibody immunoprecipitation method (S9.6-DRIP) with subsequent whole-genome sequencing, we identified 219 unique peaks of RNA-DNA hybrids in the genome of <i>Escherichia coli</i> TOP10. These peaks, which corresponded to 219 different genes, were predominantly distributed in the coding regions of the genome (88.12%). Analysis of individual genes containing RNA-DNA hybrids revealed that they encoded the enzymes involved in important energy and metabolic processes in prokaryotes, such as lipoic acid synthesis.</p>

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Genome Mapping of RNA-DNA Hybrids in Escherichia coli

  • 期刊论文

  • 发表日期：

    2025年7月15日

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  • K. Yu. Oleynikova,
  • A. S. Ruzov,
  • N. A. Zhigalova

摘要

Abstract

This is the first report on mapping the RNA-DNA hybrids in a prokaryotic genome. Using the S9.6 antibody immunoprecipitation method (S9.6-DRIP) with subsequent whole-genome sequencing, we identified 219 unique peaks of RNA-DNA hybrids in the genome of Escherichia coli TOP10. These peaks, which corresponded to 219 different genes, were predominantly distributed in the coding regions of the genome (88.12%). Analysis of individual genes containing RNA-DNA hybrids revealed that they encoded the enzymes involved in important energy and metabolic processes in prokaryotes, such as lipoic acid synthesis.