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Abstract <p>A <i>Pantoea brenneri</i> strain 3.2 with a <i>gcd</i> gene deletion was obtained to investigate how the <i>gcd</i> product (glucose dehydrogenase) contributed to ISR priming in plants. The markerless mutant strain <i>P. brenneri</i> 3.2 Δ<i>gcd</i> was created using the recombination system of the Lambda Red phage (λ Red). Inactivation of the <i>gcd</i> gene encoding glucose dehydrogenase resulted in a 2.5-fold decrease in the strain’s capacity to solubilize tricalcium phosphate when growing on solid NBRIP medium.</p>

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Inactivation of the Gene Responsible for the Synthesis of Gluconic Acid in the Genome of Pantoea brenneri

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • A. D. Suleimanova,
  • L. V. Sokolnikova,
  • D. S. Bulmakova,
  • E. A. Egorova,
  • M. R. Sharipova

摘要

Abstract

A Pantoea brenneri strain 3.2 with a gcd gene deletion was obtained to investigate how the gcd product (glucose dehydrogenase) contributed to ISR priming in plants. The markerless mutant strain P. brenneri 3.2 Δgcd was created using the recombination system of the Lambda Red phage (λ Red). Inactivation of the gcd gene encoding glucose dehydrogenase resulted in a 2.5-fold decrease in the strain’s capacity to solubilize tricalcium phosphate when growing on solid NBRIP medium.