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Abstract <p>The rat auditory sensitivity to ultrasound necessitates objectiveaudiometry in a frequency range corresponding to the evolutionaryadaptation of the acoustic analyzer in these animals. The aim ofthis study was to develop a method for investigating auditory thresholdsin rats that would physiologically match their species-specificauditory sensitivity. We used a Neuro-Audio device (Neurosoft Ltd.,Ivanovo, Russia) equipped with the Neuro-Audio.NET software, updatedfor the purpose of hearing assessment within the ultrasonic range.The study was carried out on six SPF male Wistar rats. Auditory BrainstemResponses (ABRs) were studied during monaural ipsilateral stimulationwith 8, 16, 20, and 30&#xa0;kHz tones in a free sound field. Hearingthreshold, amplitude, and peak II latency were assessed in the ABRcomplexes. For comparison, we used ABR data obtained with a conventionalmethod of delivering an 8 kHz tone stimulus via an insert earphone. Inadult rats, analysis of ABRs to 16–30 kHz tone stimulation in afree sound field environment showed hearing thresholds of 30–40dB. A morphological feature of the ABR complex at an ultrasonic stimulusintensity of 85 dB is the presence of sharply distinct peaks IIand V with an amplitude of 2.3–2.7 µV and a latency of approximately4.1–4.2 ms. An amplitude–time feature associated with pitching ina free sound field is the elongation of the peak II latency andthe appearance of a pronounced peak V. An affordable method forperforming objective audiometry in rats using ABRs in the species-specific (ultrasonic)range of auditory sensitivity and the Russian-made clinical diagnosticdevice Neuro Audio is presented. The morphology of the ABRs complexesobtained in response to 16–30 kHz tone feeds and the features associatedwith their production when studied in a free sound field are shown.</p>

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The Experience of Auditory Brainstem Response Audiometry in Wistar Rats

  • 期刊论文

  • 发表日期：

    2026年6月21日

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  • M. Yu. Naumenko,
  • A. N. Bokatyi,
  • Yu. V. Galtsev,
  • S. G. Zhuravskii

摘要

Abstract

The rat auditory sensitivity to ultrasound necessitates objectiveaudiometry in a frequency range corresponding to the evolutionaryadaptation of the acoustic analyzer in these animals. The aim ofthis study was to develop a method for investigating auditory thresholdsin rats that would physiologically match their species-specificauditory sensitivity. We used a Neuro-Audio device (Neurosoft Ltd.,Ivanovo, Russia) equipped with the Neuro-Audio.NET software, updatedfor the purpose of hearing assessment within the ultrasonic range.The study was carried out on six SPF male Wistar rats. Auditory BrainstemResponses (ABRs) were studied during monaural ipsilateral stimulationwith 8, 16, 20, and 30 kHz tones in a free sound field. Hearingthreshold, amplitude, and peak II latency were assessed in the ABRcomplexes. For comparison, we used ABR data obtained with a conventionalmethod of delivering an 8 kHz tone stimulus via an insert earphone. Inadult rats, analysis of ABRs to 16–30 kHz tone stimulation in afree sound field environment showed hearing thresholds of 30–40dB. A morphological feature of the ABR complex at an ultrasonic stimulusintensity of 85 dB is the presence of sharply distinct peaks IIand V with an amplitude of 2.3–2.7 µV and a latency of approximately4.1–4.2 ms. An amplitude–time feature associated with pitching ina free sound field is the elongation of the peak II latency andthe appearance of a pronounced peak V. An affordable method forperforming objective audiometry in rats using ABRs in the species-specific (ultrasonic)range of auditory sensitivity and the Russian-made clinical diagnosticdevice Neuro Audio is presented. The morphology of the ABRs complexesobtained in response to 16–30 kHz tone feeds and the features associatedwith their production when studied in a free sound field are shown.