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Abstract <p>Coronary artery calcification (CAC) is an important factorin the pathogenesis of coronary heart disease. Mesenchymal stemcells in epicardial adipose tissue (EAT MSCs) may represent an activecellular component contributing to the development of vascular calcification,although their role in the pathogenesis of CAC remains unexplored.Assessing the immunophenotypic features and osteoblastic potentialof EAT MSCs compared to MSCs from subcutaneous adipose tissue (SATMSCs) will allow a deeper insight into the cellular processes leadingto calcium deposition in the coronary arteries. MSC immunophenotypewas identified using flow cytometry. Expression levels of MSC surfaceantigens CD90, CD31, CD45, CD73, CD105, and HLA-DR were measured.To determine the expression levels of osteogenic differentiationgenes <i>RUNX2</i>, <i>BGLAP</i>, <i>ALPL</i>, <i>SPP1</i>, MSC cultures derived from EATand SAT were studied on days 3, 15, and 21 using real-time PCR.To assess osteogenic efficiency, the cultures were stained witha calcium-tropic dye, and calcium concentration was measured photometrically.Both EAT and SAT MSCs obtained from patients with coronary heartdisease carried CD73, CD105, and CD90 antigens on their surface, althoughthe proportion of CD73- and CD90-positive cells in the culture ofEAT MSCs was 3 and 1.3 times lower, respectively, than in that ofSAT MSCs. The expression profile of osteogenic genes in EAT MSCsshowed that this culture has a greater osteogenic potential comparedto SAT MSCs, as confirmed by a relatively high expression of <i>RUNX2</i>, <i>SP7</i>, <i>BGLAP</i>, <i>ALPL</i>, <i>SPP1</i> genes in the culture of epicardialMSCs. Conclusions: MSCs derived from EAT exhibit a higher osteogenicpotential compared to MSCs derived from SAT. This may play a keyrole in the pathogenesis of coronary artery calcification in patientswith coronary heart disease.</p>

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Comparative Evaluation of Immunophenotype and Expression Level of Osteogenic Differentiation Genes in Mesenchymal Stem Cells from Epicardial and Subcutaneous Adipose Tissue in Coronary Heart Disease

  • 期刊论文

  • 发表日期：

    2026年6月21日

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  • T. A. Slesareva,
  • E. E. Gorbatovskaya,
  • E. G. Uchasova,
  • Yu. A. Dyleva,
  • S. E. Dolmatova,
  • V. G. Matveeva,
  • E. A. Torgunakova,
  • E. V. Fanaskova,
  • I. V. Dvadtsatov,
  • I. K. Khalivopulo,
  • S. M. Gusev,
  • O. V. Gruzdeva

摘要

Abstract

Coronary artery calcification (CAC) is an important factorin the pathogenesis of coronary heart disease. Mesenchymal stemcells in epicardial adipose tissue (EAT MSCs) may represent an activecellular component contributing to the development of vascular calcification,although their role in the pathogenesis of CAC remains unexplored.Assessing the immunophenotypic features and osteoblastic potentialof EAT MSCs compared to MSCs from subcutaneous adipose tissue (SATMSCs) will allow a deeper insight into the cellular processes leadingto calcium deposition in the coronary arteries. MSC immunophenotypewas identified using flow cytometry. Expression levels of MSC surfaceantigens CD90, CD31, CD45, CD73, CD105, and HLA-DR were measured.To determine the expression levels of osteogenic differentiationgenes RUNX2, BGLAP, ALPL, SPP1, MSC cultures derived from EATand SAT were studied on days 3, 15, and 21 using real-time PCR.To assess osteogenic efficiency, the cultures were stained witha calcium-tropic dye, and calcium concentration was measured photometrically.Both EAT and SAT MSCs obtained from patients with coronary heartdisease carried CD73, CD105, and CD90 antigens on their surface, althoughthe proportion of CD73- and CD90-positive cells in the culture ofEAT MSCs was 3 and 1.3 times lower, respectively, than in that ofSAT MSCs. The expression profile of osteogenic genes in EAT MSCsshowed that this culture has a greater osteogenic potential comparedto SAT MSCs, as confirmed by a relatively high expression of RUNX2, SP7, BGLAP, ALPL, SPP1 genes in the culture of epicardialMSCs. Conclusions: MSCs derived from EAT exhibit a higher osteogenicpotential compared to MSCs derived from SAT. This may play a keyrole in the pathogenesis of coronary artery calcification in patientswith coronary heart disease.