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Abstract <p>This study examined the potential of using the pigment lipofuscin,a non‑metabolizable end product of cellular metabolism, as a biomarkerof intracellular lipid peroxidation. The study used three‑month‑oldmale Wistar rats under oxygen pressure of 5 ATA. Confocal microscopywas used to detect lipofuscin in the brain, utilizing the pigment’s autofluorescence.Morphometric analysis of sections from various hippocampal regionsrevealed lipofuscin accumulation in the rat brain following a single exposureto oxygen at 5 ATA. Lipofuscin inclusions were localized intracellularly,and the pigment distribution across different hippocampal regionswas heterogeneous. For the first time, it has been demonstratedthat neurons in the CA3 region of the hippocampus exhibit greatervulnerability to the effects of extremely high oxygen pressure and demonstratedmore intense lipofuscin accumulation compared to the CA1 region.The dynamics of neuronal lipofuscin accumulation can be considereda promising biomarker of lipid peroxidation intensity and an objectivecriterion for assessing the degree of oxidative stress in the centralnervous system. The obtained data have practical implications forthe development of methods to optimize underwater diving regimenswith high‑pressure compressed oxygen in animal models.</p>

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Lipofuscin Accumulation in Rat Hippocampal Neurons as a Marker of Oxidative Stress Induced by Extreme Hyperoxia

  • 期刊论文

  • 发表日期：

    2026年4月19日

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  • O. S. Alekseeva,
  • O. V. Kirik,
  • D. A. Sufieva,
  • D. E. Korzhevskii

摘要

Abstract

This study examined the potential of using the pigment lipofuscin,a non‑metabolizable end product of cellular metabolism, as a biomarkerof intracellular lipid peroxidation. The study used three‑month‑oldmale Wistar rats under oxygen pressure of 5 ATA. Confocal microscopywas used to detect lipofuscin in the brain, utilizing the pigment’s autofluorescence.Morphometric analysis of sections from various hippocampal regionsrevealed lipofuscin accumulation in the rat brain following a single exposureto oxygen at 5 ATA. Lipofuscin inclusions were localized intracellularly,and the pigment distribution across different hippocampal regionswas heterogeneous. For the first time, it has been demonstratedthat neurons in the CA3 region of the hippocampus exhibit greatervulnerability to the effects of extremely high oxygen pressure and demonstratedmore intense lipofuscin accumulation compared to the CA1 region.The dynamics of neuronal lipofuscin accumulation can be considereda promising biomarker of lipid peroxidation intensity and an objectivecriterion for assessing the degree of oxidative stress in the centralnervous system. The obtained data have practical implications forthe development of methods to optimize underwater diving regimenswith high‑pressure compressed oxygen in animal models.