Abstract <p>The study investigated parameters of skin microcirculationin control mice and mice with a genetic model of type 2 diabetesmellitus (T2DM). In isoflurane-anesthetized animals, the dynamicsof skin microcirculation on the right hind paw pad were measuredusing laser Doppler flowmetry at rest and in response to local heatingup to 40°C. The integral microcirculatory index (MI) was assessedas the average MI value over 15 min at rest and during the thermaltest. Adaptive spectral wavelet analysis was performed in four frequencyintervals: endothelial (0.008–0.016 Hz), neurogenic (0.016–0.05Hz), myogenic (0.05–0.2 Hz), and Mayer-wave-associated (0.2–1 Hz).The degree of linear statistical relationship between the spectralcomponents of skin microhemodynamics was determined using Spearman’srank correlation coefficients. Local heating caused a significantincrease in the integral MI in both control and T2DM animals comparedto rest. In the control group, local heating led to a significantincrease in the normalized oscillation amplitudes in the myogenicand Mayer-wave intervals, whereas in the T2DM group, they increasedonly in the Mayer-wave interval. At rest, the contribution of myogenicoscillations to the total energy of oscillations in the T2DM group,was significantly higher, while the contribution of Mayer-wave oscillationswas significantly lower compared to controls. In T2DM animals, localheating considerably reduced the number of statistically significantinternal couplings between the spectral components of microcirculatoryblood flow oscillations by 1.1 times, and their strength by 1.2times, whereas in the control group, the thermal test, on the contrary,led to an increase in both these parameters by 1.4 and 1.3 times,respectively. The obtained results demonstrate that mice with agenetic model of T2DM can be successfully employed to analyze therhythmic components of peripheral skin microhemodynamics under theinfluence of various stimuli.</p>