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Abstract <p>The accumulation of antibiotics in biocoenoses poses risksfor the development of multidrug-resistant microorganisms throughhorizontal transfer of resistance genes and metabolic changes invarious animals, including humans. These changes may affect thestructure of digestive enzymes in the gut by switching between differentclasses and isoforms of proteases, expressing new isoforms, andsignificantly impacting digestion and pathogen resistance. Thisstudy was conducted on larvae of the greater wax moth <i>Galleria mellonella</i>, which were culturedon a diet containing low doses of an antibiotic for thirty generations(R-strain). The resulting R-strain demonstrated significant resistanceof these insects to the entomopathogenic bacteria <i>Bacillus thuringiensis</i> (<i>Bt</i>). Infection of R-strain insectswith <i>Bt</i> showed a complete lossof larval susceptibility to the bacteria and a reduction in overallproteolytic enzyme activity from the first day post-treatment, primarilydue to the suppression of serine protease activity.</p>

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Comparative Analysis of Proteolytic Enzyme Activity in the Midgut of Galleria mellonella Larvae Selected for Antibiotic Resistance

  • 期刊论文

  • 发表日期：

    2025年3月1日

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  • T. N. Klementeva,
  • N. A. Kruykova,
  • D. S. Kornienko,
  • A. N. Esaulko,
  • V. V. Glupov,
  • O. V. Polenogova

摘要

Abstract

The accumulation of antibiotics in biocoenoses poses risksfor the development of multidrug-resistant microorganisms throughhorizontal transfer of resistance genes and metabolic changes invarious animals, including humans. These changes may affect thestructure of digestive enzymes in the gut by switching between differentclasses and isoforms of proteases, expressing new isoforms, andsignificantly impacting digestion and pathogen resistance. Thisstudy was conducted on larvae of the greater wax moth Galleria mellonella, which were culturedon a diet containing low doses of an antibiotic for thirty generations(R-strain). The resulting R-strain demonstrated significant resistanceof these insects to the entomopathogenic bacteria Bacillus thuringiensis (Bt). Infection of R-strain insectswith Bt showed a complete lossof larval susceptibility to the bacteria and a reduction in overallproteolytic enzyme activity from the first day post-treatment, primarilydue to the suppression of serine protease activity.