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Abstract <p>Reverse genetics often requires systems that allow intended induction of toxic genes or inactivation of vital genes at a chosen moment and/or in a chosen tissue. Cre-<i>lox</i> recombination is a common tool of genetic engineering and is used to conditionally knock out a gene, in which an exon is preliminarily flanked with <i>loxP</i> sites, or to induce expression of a gene, in which a <i>loxP-</i>flanked STOP cassette is preliminarily inserted between the promoter and the open reading frame. A mouse strain, Stra8-Cre/ER<sup>T2</sup>, was constructed to allow tamoxifen induction of Cre-<i>lox</i> recombination in spermatocytes. Construction was performed using a 400-bp promoter of the <i>Stra8</i> gene, whose protein product STRA8 initiates the transition from mitosis to meiosis in response to a growing concentration of retinoic acid in the cell. The strain will be further used to study the regulatory mechanisms of meiosis.</p>

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Generation of Stra8-Cre/ERT2 Mouse Activator Strain for Inducible Cre-lox Recombination in Spermatocytes

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • L. A. Ilchuk,
  • M. V. Kubekina,
  • Yu. D. Okulova,
  • E. N. Korshunov,
  • M. A. Filatov,
  • A. V. Bruter,
  • Yu. Yu. Silaeva

摘要

Abstract

Reverse genetics often requires systems that allow intended induction of toxic genes or inactivation of vital genes at a chosen moment and/or in a chosen tissue. Cre-lox recombination is a common tool of genetic engineering and is used to conditionally knock out a gene, in which an exon is preliminarily flanked with loxP sites, or to induce expression of a gene, in which a loxP-flanked STOP cassette is preliminarily inserted between the promoter and the open reading frame. A mouse strain, Stra8-Cre/ERT2, was constructed to allow tamoxifen induction of Cre-lox recombination in spermatocytes. Construction was performed using a 400-bp promoter of the Stra8 gene, whose protein product STRA8 initiates the transition from mitosis to meiosis in response to a growing concentration of retinoic acid in the cell. The strain will be further used to study the regulatory mechanisms of meiosis.