Scholarlink

Abstract <p>Fullerenol C<sub>60</sub>(OH)<sub>22–24</sub> was tested for its effect on the viability of human peripheral blood monocytes, and its adhesion/internalization by monocytes was studied. Nanoparticles used at concentrations of 0.25, 0.5, 2.5, 5, 12.5, 25, 50, 100, and 200 μg/mL were incubated with human blood mononuclear cells for 24, 48, and 72 h (5% CO<sub>2</sub> at 37°C). Monocyte viability and fullerenol fluorescence intensity in the monocyte gate were then assessed by flow cytometry. Nanoparticles were adsorbed/internalized by cells in proportion to their concentration and incubation time. Fullerenol C<sub>60</sub>(OH)<sub>22–24</sub> used at 200 μg/mL reduced the monocyte viability on average by 17, 30, and 28% after 24, 48, and 72 h, respectively. Cell survival inversely correlated with nanoparticle uptake intensity. The concentrations ≤100 μg/mL did not exhibit cytotoxicity even after prolonged exposure, supporting biomedical applicability of fullerenol.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cytotoxicity of Fullerenol C60(OH)22–24 to Human Peripheral Blood Monocytes

  • 期刊论文

  • 发表日期：

    2025年10月1日

查看全文
  • V. P. Timganova,
  • M. S. Bochkova,
  • S. S. Lazarev,
  • M. D. Dolgikh,
  • D. I. Usanina,
  • S. A. Zamorina,
  • M. B. Rayev

摘要

Abstract

Fullerenol C60(OH)22–24 was tested for its effect on the viability of human peripheral blood monocytes, and its adhesion/internalization by monocytes was studied. Nanoparticles used at concentrations of 0.25, 0.5, 2.5, 5, 12.5, 25, 50, 100, and 200 μg/mL were incubated with human blood mononuclear cells for 24, 48, and 72 h (5% CO2 at 37°C). Monocyte viability and fullerenol fluorescence intensity in the monocyte gate were then assessed by flow cytometry. Nanoparticles were adsorbed/internalized by cells in proportion to their concentration and incubation time. Fullerenol C60(OH)22–24 used at 200 μg/mL reduced the monocyte viability on average by 17, 30, and 28% after 24, 48, and 72 h, respectively. Cell survival inversely correlated with nanoparticle uptake intensity. The concentrations ≤100 μg/mL did not exhibit cytotoxicity even after prolonged exposure, supporting biomedical applicability of fullerenol.