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Abstract <p>Polyclonal antibodies specific to the N-sequence (76–289 aa) of obscurin of the long-tailed ground squirrel (<i>Urocitellus undulatus</i>) were used to study the isoform composition of this protein in two striated muscles: the myocardium of the left ventricle and M. gastrocnemius medialis. One low-molecular-weight isoform of obscurin with a molecular weight of 115 kDa has been identified. No other isoforms of obscurin, including giant A- and B-isoforms, have been detected. Seasonal changes in the levels of the low-molecular-weight obscurin isoform visualized in the cardiac and skeletal muscles of long-tailed ground squirrels were not observed. Further research could focus on sequencing the <i>obscn</i> gene of long-tailed ground squirrels and analyzing obscurin transcripts to obtain antibodies specific to the giant isoforms of this protein.</p>

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Production of Polyclonal Antibodies Specific to Obscurin of the Long-Tailed Ground Squirrel Urocitellus undulatus: Problems and Prospects

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • T. A. Uryupina,
  • S. S. Popova,
  • G. Z. Mikhailova,
  • L. G. Bobyleva,
  • A. D. Ulanova,
  • A. G. Bobylev,
  • S. N. Udaltsov,
  • N. M. Zakharova,
  • I. M. Vikhlyantsev

摘要

Abstract

Polyclonal antibodies specific to the N-sequence (76–289 aa) of obscurin of the long-tailed ground squirrel (Urocitellus undulatus) were used to study the isoform composition of this protein in two striated muscles: the myocardium of the left ventricle and M. gastrocnemius medialis. One low-molecular-weight isoform of obscurin with a molecular weight of 115 kDa has been identified. No other isoforms of obscurin, including giant A- and B-isoforms, have been detected. Seasonal changes in the levels of the low-molecular-weight obscurin isoform visualized in the cardiac and skeletal muscles of long-tailed ground squirrels were not observed. Further research could focus on sequencing the obscn gene of long-tailed ground squirrels and analyzing obscurin transcripts to obtain antibodies specific to the giant isoforms of this protein.