Scholarlink

<p>Covalent complexes of prourokinase, miniplasmin, and thrombin with QD nanoparticles containing aldehyde, amino, and carboxyl groups on the surface were obtained. It was shown that under the selected immobilization conditions, the listed enzymes immobilized on QD nanoparticles fully retained their enzymatic activity and the formed complexes were stable colloids. The coating density of nanoparticles with enzymes reached 50–100 molecules per particle. The immobilization of enzymes on carboxylic nanoparticles was performed using cabodiimide. Unlike the technology with carboxyl groups, the immobilization of enzymes on aldehyde nanoparticles does not require the use of carbodiimide to form a covalent complex and does not require a procedure for washing reagents before adding enzymes to nanoparticles, which improves the reproducibility of the immobilization procedure. It has been shown that particles with immobilized thrombin, when incubated with fibrinogen or blood plasma, form microemboli, which in vitro are completely destroyed by plasmin in the physiological concentration of the latter.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A Method for Obtaining Covalent Conjugates of QD Particles with Enzymes Involved in Thrombosis and Thrombolysis

  • 期刊论文

  • 发表日期：

    2025年10月1日

查看全文
  • L. P. Savochkina,
  • T. N. Belyanko,
  • Z. I. Tsokolaeva,
  • R. Sh. Bibilashvili

摘要

Covalent complexes of prourokinase, miniplasmin, and thrombin with QD nanoparticles containing aldehyde, amino, and carboxyl groups on the surface were obtained. It was shown that under the selected immobilization conditions, the listed enzymes immobilized on QD nanoparticles fully retained their enzymatic activity and the formed complexes were stable colloids. The coating density of nanoparticles with enzymes reached 50–100 molecules per particle. The immobilization of enzymes on carboxylic nanoparticles was performed using cabodiimide. Unlike the technology with carboxyl groups, the immobilization of enzymes on aldehyde nanoparticles does not require the use of carbodiimide to form a covalent complex and does not require a procedure for washing reagents before adding enzymes to nanoparticles, which improves the reproducibility of the immobilization procedure. It has been shown that particles with immobilized thrombin, when incubated with fibrinogen or blood plasma, form microemboli, which in vitro are completely destroyed by plasmin in the physiological concentration of the latter.