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<p>We demonstrated the temperature heterogeneity of an electrophoretic solution, which is dynamically changing during electrophoresis in the gel platform area, previously. In order to understand the reasons for this effect, we used U-shaped inserts in the wells of the electrophoretic chamber, protruding above the surface of the electrophoretic solution, but not affecting the current flow. The study showed that the migration of heated masses of solution is caused by different rates of hydrogen and oxygen production on platinum electrodes. Using the technique of real-time thermal imaging it was demonstrated that in the presence of U-shaped inserts heating was uniform up to 7°С throughout the entire volume of the solution located above the gel platform. Heating of the liquid in the well area occurred with lower dynamics and was not identical. The heating of the liquid in the cathode well occurs insignificantly (only 1.5°С), while at the anode the increase is 2.5°С. We believe that temperature homogeneity will be of significant importance for reducing the variability of the results obtained by the Comet assay (DNA comet) method on preparations subjected to alkaline electrophoresis.</p>

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Thermal Processes Associated with Electrophoresis under Conditions of High Ionic Strength

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • N. P. Sirota,
  • E. P. Khizhnyak,
  • L. N. Khizhnyak,
  • T. V. Sirota,
  • E. A. Kuznetsova

摘要

We demonstrated the temperature heterogeneity of an electrophoretic solution, which is dynamically changing during electrophoresis in the gel platform area, previously. In order to understand the reasons for this effect, we used U-shaped inserts in the wells of the electrophoretic chamber, protruding above the surface of the electrophoretic solution, but not affecting the current flow. The study showed that the migration of heated masses of solution is caused by different rates of hydrogen and oxygen production on platinum electrodes. Using the technique of real-time thermal imaging it was demonstrated that in the presence of U-shaped inserts heating was uniform up to 7°С throughout the entire volume of the solution located above the gel platform. Heating of the liquid in the well area occurred with lower dynamics and was not identical. The heating of the liquid in the cathode well occurs insignificantly (only 1.5°С), while at the anode the increase is 2.5°С. We believe that temperature homogeneity will be of significant importance for reducing the variability of the results obtained by the Comet assay (DNA comet) method on preparations subjected to alkaline electrophoresis.