Scholarlink

Abstract <p>The main purpose of this work was to study the stoichiometry of the interaction of cytochrome C (CytC) with tetraoleoyl (TOCL)- or tetramyristoyl (TMCL)-cardiolipin—components of CytC-phospholipid complexes that play one of the main roles in apoptotic processes. The study was carried out using fluorescently labeled phosphatidylcholine (BODIPY-PC). The fluorescence quenching of the BODIPY-PC probe by cytochrome C was measured. Calculations of the fluorescence quenching results enabled to determine the stoichiometry of the formed CytC–phospholipid complexes. The stoichiometry for TOCL-containing complexes corresponded to a TOCL : CytC ratio of 25 ± 7, and that for TMCL-containing complexes—to a TMCL : CytC ratio of 7 ± 2. The obtained results may indicate a difference in the affinity of cytochrome C to saturated and unsaturated types of cardiolipin despite the same electrostatic characteristics of the molecules. It can be expected that the discovered features of cytochrome C interaction with cardiolipin will eventually be able to determine the intensity and direction of apoptotic reactions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Stoichiometry of Formation of Physiologically Active Cytochrome C–Cardiolipin Complexes

  • 期刊论文

  • 发表日期：

    2025年2月1日

查看全文
  • G. O. Stepanov,
  • G. K. Vladimirov,
  • I. V. Kirilina,
  • A. P. Guselnikova,
  • V. I. Tikhonova,
  • P. D. Vinogradova,
  • A. N. Osipov,
  • Yu. A. Vladimirov

摘要

Abstract

The main purpose of this work was to study the stoichiometry of the interaction of cytochrome C (CytC) with tetraoleoyl (TOCL)- or tetramyristoyl (TMCL)-cardiolipin—components of CytC-phospholipid complexes that play one of the main roles in apoptotic processes. The study was carried out using fluorescently labeled phosphatidylcholine (BODIPY-PC). The fluorescence quenching of the BODIPY-PC probe by cytochrome C was measured. Calculations of the fluorescence quenching results enabled to determine the stoichiometry of the formed CytC–phospholipid complexes. The stoichiometry for TOCL-containing complexes corresponded to a TOCL : CytC ratio of 25 ± 7, and that for TMCL-containing complexes—to a TMCL : CytC ratio of 7 ± 2. The obtained results may indicate a difference in the affinity of cytochrome C to saturated and unsaturated types of cardiolipin despite the same electrostatic characteristics of the molecules. It can be expected that the discovered features of cytochrome C interaction with cardiolipin will eventually be able to determine the intensity and direction of apoptotic reactions.