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Abstract <p>Changes in the Na<sup>+</sup> and K<sup>+</sup> intracellular concentrations affect expression of the <i>FOS</i> gene. Here, we obtained a genetic construct coding for the TurboGFP-dest1 protein under control of the human <i>FOS</i> promoter (−549 to +155) and studied its expression in HEK293T cells exposed to monovalent metal cations. Amplification of the <i>FOS</i> promoter sequence from genomic DNA was efficient only in the presence of Li<sup>+</sup> ions. Incubation of cells with ouabain or in a medium containing Li<sup>+</sup> ions instead of Na<sup>+</sup> ions caused intracellular accumulation of Na<sup>+</sup> and Li<sup>+</sup> ions, respectively. In addition, both stimuli increased the levels of endogenous <i>FOS</i> mRNA and the average fluorescence intensity of TurboGFP-dest1 in transfected cells. The mRNA levels of TurboGFP-dest1 were significantly higher than the <i>FOS</i> mRNA levels and were little affected by the stimuli.</p>

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FOS Promoter is Overactive Outside of Genome Context and Weakly Regulated by Changes in the Na+i/K+i Ratio

  • 期刊论文

  • 发表日期：

    2025年5月1日

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  • Andrey M. Gorbunov,
  • Dmitrii A. Fedorov,
  • Olga E. Kvitko,
  • Olga D. Lopina,
  • Elizaveta A. Klimanova

摘要

Abstract

Changes in the Na+ and K+ intracellular concentrations affect expression of the FOS gene. Here, we obtained a genetic construct coding for the TurboGFP-dest1 protein under control of the human FOS promoter (−549 to +155) and studied its expression in HEK293T cells exposed to monovalent metal cations. Amplification of the FOS promoter sequence from genomic DNA was efficient only in the presence of Li+ ions. Incubation of cells with ouabain or in a medium containing Li+ ions instead of Na+ ions caused intracellular accumulation of Na+ and Li+ ions, respectively. In addition, both stimuli increased the levels of endogenous FOS mRNA and the average fluorescence intensity of TurboGFP-dest1 in transfected cells. The mRNA levels of TurboGFP-dest1 were significantly higher than the FOS mRNA levels and were little affected by the stimuli.