Scholarlink

Abstract <p>Evaluation of sperm-specific gene expression is a potential resource for diagnostics of impaired spermiogenesis, cryoresistance, and the fertility of these germ cells. These are important indicators of sperm quality, which should be taken into account when selecting breeding bulls for collecting semen intended for cryopreservation. Here, a correlation analysis of the expression levels of the <i>AQP7, STK35, IFT27,</i> and <i>PRM1</i> genes with sperm quality indicators in native and decryopreserved sperm of Holstein bulls was carried out. We found a highly reliable positive correlation between the expression of <i>AQP7, ITF27,</i> and <i>PRM1</i> with a number of spermatozoa viability indicators and a negative one with indicators characterizing the loss of their functionality. The results obtained can be used for developing transcriptional biomarkers of sperm quality in breeding bulls based on the parameters of cryotolerance and the fertilizing potential of spermatozoa.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Semen Quality of Holstein Bulls and Expression of the AQP7, STK35, ITF27, and PRM1 Genes: Correlation Analysis

  • 期刊论文

  • 发表日期：

    2025年12月1日

查看全文
  • O. Yu. Barkova,
  • D. A. Starikova,
  • I. V. Chistyakova,
  • N. V. Pleshanov

摘要

Abstract

Evaluation of sperm-specific gene expression is a potential resource for diagnostics of impaired spermiogenesis, cryoresistance, and the fertility of these germ cells. These are important indicators of sperm quality, which should be taken into account when selecting breeding bulls for collecting semen intended for cryopreservation. Here, a correlation analysis of the expression levels of the AQP7, STK35, IFT27, and PRM1 genes with sperm quality indicators in native and decryopreserved sperm of Holstein bulls was carried out. We found a highly reliable positive correlation between the expression of AQP7, ITF27, and PRM1 with a number of spermatozoa viability indicators and a negative one with indicators characterizing the loss of their functionality. The results obtained can be used for developing transcriptional biomarkers of sperm quality in breeding bulls based on the parameters of cryotolerance and the fertilizing potential of spermatozoa.