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Abstract <p>The culturing systems of cells in three-dimensional space have become central to the study of interactions between different cell lines. These technologies have become crucial tools in modern cell biology research and have found practical application by eliminating limitations inherent to monolayer cultures and achieving more accurate reproduction of cellular functions. The use of three-dimensional (3D) cell models not only reduces the labor and cost of research but also decreases the amount of work involving laboratory animals. However, a universal method for 3D culturing that combines simultaneously high efficiency and good reproducibility, and at the same time does not require high costs has not yet been developed. In this regard, the search for new alternative strategies aimed at improving the technology of 3D cell culturing continues. This review presents data of Russian and foreign scientific publications on methods for creating 3D cell systems and analyzes their advantages and disadvantages. The necessity of modification of existing methods and development of new ones for 3D cell culturing is discussed.</p>

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3D-Cell Culture Systems: Current State

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • I. A. Ivashchenko,
  • W. W. Wasielewski,
  • O. S. Fedotova,
  • V. N. Slautin,
  • A. V. Semenov

摘要

Abstract

The culturing systems of cells in three-dimensional space have become central to the study of interactions between different cell lines. These technologies have become crucial tools in modern cell biology research and have found practical application by eliminating limitations inherent to monolayer cultures and achieving more accurate reproduction of cellular functions. The use of three-dimensional (3D) cell models not only reduces the labor and cost of research but also decreases the amount of work involving laboratory animals. However, a universal method for 3D culturing that combines simultaneously high efficiency and good reproducibility, and at the same time does not require high costs has not yet been developed. In this regard, the search for new alternative strategies aimed at improving the technology of 3D cell culturing continues. This review presents data of Russian and foreign scientific publications on methods for creating 3D cell systems and analyzes their advantages and disadvantages. The necessity of modification of existing methods and development of new ones for 3D cell culturing is discussed.