Scholarlink

Abstract <p>The conditions of recombinant ɑ-amylase production in an induction-free cultivation system based on <i>Bacillus mojavensis</i> are described. Fermentation was carried out in pilot fermenters under deep cultivation, which ensured the production of experimental batches in the quantities necessary for comprehensive studies of the samples obtained. Gradual accumulation of the recombinant enzyme in the bioreactor reaches a maximum after 31 hours. Some biochemical parameters of the recombinant enzyme were studied. Its thermostability was established, and the pH optimum of activity manifestation was determined—the maximum efficiency of substrate hydrolysis was observed when the sample was preheated for 30 min at a temperature of 55°C and pH equal to 7.2. An inversely proportional relationship between the change in the level of amylolytic activity and the amount of calcium chloride added during cultivation was revealed. The possibility of creating strains producing recombinant ɑ-amylase on the basis of <i>B. mojavensis</i> strain BDV-1 was demonstrated.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Production of α-Amylase Bacillus amyloliquefaciens in the Expression System of Bacillus mojavensis and Properties of the Recombinant Enzyme

  • 期刊论文

  • 发表日期：

    2025年12月9日

查看全文
  • V. Yu. Chirkova,
  • D. N. Shcherbakov,
  • P. V. Kolosov,
  • M. V. Shirmanov,
  • E. A. Kolosova,
  • A. V. Malkova,
  • E. A. Sharlaeva,
  • A. N. Irkitova,
  • I. Yu. Evdokimov

摘要

Abstract

The conditions of recombinant ɑ-amylase production in an induction-free cultivation system based on Bacillus mojavensis are described. Fermentation was carried out in pilot fermenters under deep cultivation, which ensured the production of experimental batches in the quantities necessary for comprehensive studies of the samples obtained. Gradual accumulation of the recombinant enzyme in the bioreactor reaches a maximum after 31 hours. Some biochemical parameters of the recombinant enzyme were studied. Its thermostability was established, and the pH optimum of activity manifestation was determined—the maximum efficiency of substrate hydrolysis was observed when the sample was preheated for 30 min at a temperature of 55°C and pH equal to 7.2. An inversely proportional relationship between the change in the level of amylolytic activity and the amount of calcium chloride added during cultivation was revealed. The possibility of creating strains producing recombinant ɑ-amylase on the basis of B. mojavensis strain BDV-1 was demonstrated.