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Abstract <p>Cryopreservation results in a significant reduction in the fertilizing properties of sperm, but genetic variations among different animal species affect the survival of germ cells after cryopreservation. Searching for specific genetic markers can be an effective way to select bulls before semen collection for cryopreservation. A correlation analysis of the expression level of candidate genes, <i>HSPA1A</i>, <i>HSBPA1</i>, <i>AFAF</i>, <i>AQP3,</i> and <i>AQP11</i>, with sperm quality indicators in native and decryopreserved sperm of Holstein bulls was carried out. Highly reliable positive correlations of the studied gene transcripts with a number of indicators of sperm viability and negative ones with indicators characterizing the loss of their functionality were revealed. The results of this study can be used to develop transcriptional biomarkers of bull sperm quality, namely based on the parameters of cryoresistance and the fertilizing potential of sperm.</p>

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HSPA1A, HSBPA1, AFAF, AQP3, and AQP11 Transcripts as Potential Markers of Sperm Quality in Holstein Bulls

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • O. Yu. Barkova,
  • D. A. Starikova,
  • I. V. Chistyakova,
  • A. P. Dysin,
  • N. V. Pleshanov

摘要

Abstract

Cryopreservation results in a significant reduction in the fertilizing properties of sperm, but genetic variations among different animal species affect the survival of germ cells after cryopreservation. Searching for specific genetic markers can be an effective way to select bulls before semen collection for cryopreservation. A correlation analysis of the expression level of candidate genes, HSPA1A, HSBPA1, AFAF, AQP3, and AQP11, with sperm quality indicators in native and decryopreserved sperm of Holstein bulls was carried out. Highly reliable positive correlations of the studied gene transcripts with a number of indicators of sperm viability and negative ones with indicators characterizing the loss of their functionality were revealed. The results of this study can be used to develop transcriptional biomarkers of bull sperm quality, namely based on the parameters of cryoresistance and the fertilizing potential of sperm.