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Abstract— <p>The work has demonstrated that high-molecular chitosan enhances the lytic effect of lysostaphin on <i>Staphylococcus aureus</i> cells. More efficient lysis of bacterial cell walls by lysostaphin in the presence of chitosan made it possible to extract larger amounts of high molecular weight DNA suitable for sequencing from bacterial cells.</p>

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Chitosan-Based Enzymatic Cell Wall Lysis Modulator for Bacterial DNA Isolation

  • 期刊论文

  • 发表日期：

    2026年4月16日

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  • K. Jasem,
  • R. Z. Khayrullin,
  • S. N. Kulikov

摘要

Abstract—

The work has demonstrated that high-molecular chitosan enhances the lytic effect of lysostaphin on Staphylococcus aureus cells. More efficient lysis of bacterial cell walls by lysostaphin in the presence of chitosan made it possible to extract larger amounts of high molecular weight DNA suitable for sequencing from bacterial cells.