Abstract— <p>In the context of growing antibiotic resistance, the possibility of creating non-toxic and biodegradable film coatings based on chitosan with modulated antibacterial properties is highly relevant. We have analyzed 270 samples of biopolymer films based on chitosan cross-linked with genipin with molecular weights of 60, 190, and 320 kDa. Suspensions of 30 clinical strains of <i>Staphilococcus aureus</i> (<i>n</i> = 10), <i>Escherichia coli</i> (<i>n</i>&#xa0;= 10), and <i>Candida albicans</i> (<i>n</i> = 10) microorganisms at a concentration of 10³ CFU/mL have been used to evaluate the antibacterial activity of the biopolymer chitosan films (CF). Depending on the molecular weight of chitosan and the contact time of the biopolymer films with <i>S. aureus, E. coli, and C. albicans</i>, negative dynamics in the number of viable colonies have been revealed. These results suggest that chitosan-based biopolymers hold promise for the development of medical tools with programmable antibacterial and antifungal properties for practical medical use.</p>