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Abstract— <p>A new method is proposed for early identification of the causal agent of grapevine downy mildew <i>Plasmopara viticola</i>, based on a real-time quantitative PCR (real-time PCR) method using the fluorescent dye SYBR Green I. Six pairs of primers were developed for real-time PCR for identification of <i>P. viticola</i>; among them, the primer pair PvITS1_2-real-s/a demonstrated the highest efficiency for early detection of grapevine downy mildew. Moreover, a positive correlation (<i>R</i> = 0.86) was demonstrated when comparing the results of real-time PCR with the primers PvITS1_2-real-s/a with data on metataxonomic analysis of the distribution of <i>P. viticola</i> among plants of Far Eastern grape species and varieties. Thus, real-time PCR with the primer pair PvITS1_2-real-s/a is a cheap and efficient method for early detection and monitoring of asymptomatic <i>P. viticola</i> infections. The developed method can be a basis for predicting epidemics of grapevine downy mildew and its control in vineyards.</p>

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Identification of the Causal Agent of Grapevine Downy Mildew Plasmopara viticola Based on Quantitative PCR

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月1日

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  • N. N. Nityagovsky,
  • A. A. Dneprovskaya,
  • A. A. Ananev,
  • K. V. Kiselev,
  • O. A. Aleynova

摘要

Abstract—

A new method is proposed for early identification of the causal agent of grapevine downy mildew Plasmopara viticola, based on a real-time quantitative PCR (real-time PCR) method using the fluorescent dye SYBR Green I. Six pairs of primers were developed for real-time PCR for identification of P. viticola; among them, the primer pair PvITS1_2-real-s/a demonstrated the highest efficiency for early detection of grapevine downy mildew. Moreover, a positive correlation (R = 0.86) was demonstrated when comparing the results of real-time PCR with the primers PvITS1_2-real-s/a with data on metataxonomic analysis of the distribution of P. viticola among plants of Far Eastern grape species and varieties. Thus, real-time PCR with the primer pair PvITS1_2-real-s/a is a cheap and efficient method for early detection and monitoring of asymptomatic P. viticola infections. The developed method can be a basis for predicting epidemics of grapevine downy mildew and its control in vineyards.