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<p>Current approaches to detect analytes use biomolecules or single cells and thus do not harness the computational power of endogenous algorithms present in micro-organs. Consequently, current on-line detection of glucose does not allow an autonomous artificial pancreas. In contrast, monitoring a few electrogenic pancreatic islets may provide a more appropriate read-out. Indeed, upon stimulation islets react with so-called slow potentials (SP) and their amplitude reflects the degree of intercellular coupling. We have now developed a microfluidic microelectrode chip containing a few islets and linked to interstitial fluids in live rats by microdialysis. Blood and dialysate glucose were determined concomitantly with ex-vivo islet electrical activity during an intraperitoneal glucose or insulin challenge. Blood glucose was tightly correlated to islet SP frequency, and to a lesser degree to SP amplitudes. This demonstrates the feasibility and usefulness of microorgan-based biosensors which may also be of interest in the therapy of diabetes.</p>

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A micro-organ based microfluidic biosensor for continuous monitoring of glucose levels in vivo

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月19日

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  • Emilie Puginier,
  • Antoine Pirog,
  • Florence Poulletier de Gannes,
  • Julien Gaitan,
  • Annabelle Hurtier,
  • Dorian Chapeau,
  • Marie Monchablon,
  • Matthieu Raoux,
  • Sylvie Renaud,
  • Jochen Lang

摘要

Current approaches to detect analytes use biomolecules or single cells and thus do not harness the computational power of endogenous algorithms present in micro-organs. Consequently, current on-line detection of glucose does not allow an autonomous artificial pancreas. In contrast, monitoring a few electrogenic pancreatic islets may provide a more appropriate read-out. Indeed, upon stimulation islets react with so-called slow potentials (SP) and their amplitude reflects the degree of intercellular coupling. We have now developed a microfluidic microelectrode chip containing a few islets and linked to interstitial fluids in live rats by microdialysis. Blood and dialysate glucose were determined concomitantly with ex-vivo islet electrical activity during an intraperitoneal glucose or insulin challenge. Blood glucose was tightly correlated to islet SP frequency, and to a lesser degree to SP amplitudes. This demonstrates the feasibility and usefulness of microorgan-based biosensors which may also be of interest in the therapy of diabetes.