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<p>In most metazoans, centrosome elimination during oogenesis ensures accurate centriole inheritance in the zygote, yet the molecular mechanisms remain poorly understood. Here, we reveal a critical role for controlled SAS-6 phosphorylation in centrosome dynamics during oogenesis. Centrioles disassemble during late meiotic prophase, while the cartwheel protein SAS-6 exhibits dynamic behavior in early meiotic prophase. Purified SAS-6 undergoes phase separation in vitro, and overexpressed SAS-6 forms droplets in cells. Mass spectrometry and kinase assays reveal that SAS-6 is phosphorylated at its C-terminus in cells and in vivo, with CDK-1 identified as a direct kinase. This phosphorylation inhibits SAS-6 phase separation and weakens interactions between centriolar proteins. SAS-6 degradation confirms its role in centrosome stability, and CDK-1 activity is required for timely centriole disassembly. Phospho-mimetic and phospho-deficient mutants demonstrate that dynamic SAS-6 phosphorylation is essential for centrosome assembly and elimination. We propose that the disordered C-terminus of SAS-6 facilitates cartwheel stacking via multivalent weak interactions, promoting centriole stability. Phosphorylation disrupts these interactions, impairing centrosome duplication and promoting elimination during oogenesis.</p>

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Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月23日

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  • Feifei Qi,
  • Shanshan Yin,
  • Xiangrui Yang,
  • Ning Ju,
  • Bohan Liu,
  • Xing Zhang,
  • Zixuan Zhu,
  • Li Ji,
  • Fuxin Zhang,
  • Li Zhao,
  • Ruoxi Wang,
  • Min Liu,
  • Liangran Zhang,
  • Huijie Zhao,
  • Jun Zhou,
  • Jinmin Gao

摘要

In most metazoans, centrosome elimination during oogenesis ensures accurate centriole inheritance in the zygote, yet the molecular mechanisms remain poorly understood. Here, we reveal a critical role for controlled SAS-6 phosphorylation in centrosome dynamics during oogenesis. Centrioles disassemble during late meiotic prophase, while the cartwheel protein SAS-6 exhibits dynamic behavior in early meiotic prophase. Purified SAS-6 undergoes phase separation in vitro, and overexpressed SAS-6 forms droplets in cells. Mass spectrometry and kinase assays reveal that SAS-6 is phosphorylated at its C-terminus in cells and in vivo, with CDK-1 identified as a direct kinase. This phosphorylation inhibits SAS-6 phase separation and weakens interactions between centriolar proteins. SAS-6 degradation confirms its role in centrosome stability, and CDK-1 activity is required for timely centriole disassembly. Phospho-mimetic and phospho-deficient mutants demonstrate that dynamic SAS-6 phosphorylation is essential for centrosome assembly and elimination. We propose that the disordered C-terminus of SAS-6 facilitates cartwheel stacking via multivalent weak interactions, promoting centriole stability. Phosphorylation disrupts these interactions, impairing centrosome duplication and promoting elimination during oogenesis.