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<p>Plant plasma membrane proteins are organized into distinct domains; however, the principles that govern this spatial organization are not fully understood. HYPERSENSITIVE-INDUCED REACTION (HIR) proteins are plant-specific members of the stomatin/prohibitin/ flotillin/HflK/C (SPFH) family implicated in membrane organization. Arabidopsis thaliana HIR2 interacts with multiple plasma membrane proteins, including receptor kinases such as BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE (BAK1)-INTERACTING RECEPTORS 2 and 3 (BIR2 and 3) and BAK1. These interactions connect HIR2 to BAK1-mediated signaling pathways, as evidenced by impaired growth and immunity phenotypes in hir2 mutants. HIR2 is anchored to the inner leaflet of the plasma membrane through a hydrophobic domain and S-acylation. Single-particle-tracking photoactivated localization microscopy (sptPALM) revealed that HIR2 affects BAK1 mobility and clustering, exemplifying a role in regulating plasma membrane dynamics. Structural modeling with AlphaFold 3 predicts a multimeric cup-like assembly for HIR2, consistent with high molecular weight complexes identified through blue-native PAGE. These findings indicate that HIR2 contributes to the formation of membrane sub-compartments, providing a potential structural framework for spatial membrane organization that influences the dynamics and function of membrane-resident receptors.</p>

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The Arabidopsis SPFH protein HIR2 modulates receptor signaling and plasma membrane organization

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月29日

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  • Hannah Weber,
  • Alexandra Ehinger,
  • Dagmar Kolb,
  • Vahid Fallahzadeh-Mamaghani,
  • Thierry Halter,
  • Mauricio P Contreras,
  • Mirita Franz,
  • Nora Schäfer,
  • Sven zur Oven-Krockhaus,
  • Julien Gronnier,
  • Claudia Oecking,
  • Cyril Zipfel,
  • Klaus Harter,
  • Birgit Kemmerling

摘要

Plant plasma membrane proteins are organized into distinct domains; however, the principles that govern this spatial organization are not fully understood. HYPERSENSITIVE-INDUCED REACTION (HIR) proteins are plant-specific members of the stomatin/prohibitin/ flotillin/HflK/C (SPFH) family implicated in membrane organization. Arabidopsis thaliana HIR2 interacts with multiple plasma membrane proteins, including receptor kinases such as BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE (BAK1)-INTERACTING RECEPTORS 2 and 3 (BIR2 and 3) and BAK1. These interactions connect HIR2 to BAK1-mediated signaling pathways, as evidenced by impaired growth and immunity phenotypes in hir2 mutants. HIR2 is anchored to the inner leaflet of the plasma membrane through a hydrophobic domain and S-acylation. Single-particle-tracking photoactivated localization microscopy (sptPALM) revealed that HIR2 affects BAK1 mobility and clustering, exemplifying a role in regulating plasma membrane dynamics. Structural modeling with AlphaFold 3 predicts a multimeric cup-like assembly for HIR2, consistent with high molecular weight complexes identified through blue-native PAGE. These findings indicate that HIR2 contributes to the formation of membrane sub-compartments, providing a potential structural framework for spatial membrane organization that influences the dynamics and function of membrane-resident receptors.