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<p>This study investigates the electric field distribution of an extended class of diffraction-free Airy beams, termed Airy-like beams, aiming to identify an optimal configuration that balances field of view (FOV) and axial resolution for light-sheet fluorescence microscopy (LSFM). Based on the Olver function framework, we derived an analytical expression of the electric field through phase term superposition and established a mapping relationship between the pupil function and electric field distribution. Numerical simulations and modulation transfer function (MTF) analysis revealed that the optimal Airy-like beam form (<i>m</i> = 0 + 1) provides a more balanced trade-off between axial resolution and field of view than conventional Airy beams. Fluorescent microsphere experiments further demonstrate an axial resolution improvement of approximately 17.3% compared with conventional Airy beams. Validation through light-sheet imaging simulations and fluorescent microsphere experiments confirmed its improved imaging performance, particularly in peripheral regions. We further applied the optimized Airy-like beam to visualize cerebral vasculature in large-scale brain tissues, demonstrating its capability to resolve sophisticated structures consistently across an extended FOV. This result could be a clue for Airy-like beam design in advancing LSFM.</p>

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Investigation of Propagation Properties of Airy-like Beams for Light-Sheet Fluorescence Microscopy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月29日

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  • Yanni Wang,
  • Qizhong Wang,
  • Jianmin Ding,
  • Rong Zhao,
  • Siyu Ao,
  • Xin Xiong,
  • Shibo Li,
  • Di Ma,
  • Huizhen Ma,
  • Zifan Wang,
  • Zixin Yang,
  • Hao Wang,
  • Xiao-Dong Zhang,
  • Pengfei Liu

摘要

This study investigates the electric field distribution of an extended class of diffraction-free Airy beams, termed Airy-like beams, aiming to identify an optimal configuration that balances field of view (FOV) and axial resolution for light-sheet fluorescence microscopy (LSFM). Based on the Olver function framework, we derived an analytical expression of the electric field through phase term superposition and established a mapping relationship between the pupil function and electric field distribution. Numerical simulations and modulation transfer function (MTF) analysis revealed that the optimal Airy-like beam form (m = 0 + 1) provides a more balanced trade-off between axial resolution and field of view than conventional Airy beams. Fluorescent microsphere experiments further demonstrate an axial resolution improvement of approximately 17.3% compared with conventional Airy beams. Validation through light-sheet imaging simulations and fluorescent microsphere experiments confirmed its improved imaging performance, particularly in peripheral regions. We further applied the optimized Airy-like beam to visualize cerebral vasculature in large-scale brain tissues, demonstrating its capability to resolve sophisticated structures consistently across an extended FOV. This result could be a clue for Airy-like beam design in advancing LSFM.