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<p>A virus stock of a cloned infectious DNA of simian foamy virus serotype 1 (SFV-1) was obtained in <i>M. dunni</i> cells (designated as pSFV-MD). The kinetics of replication were similar to the parent uncloned SFV-MD stock in <i>M. dunni</i> cells and in FRhK-4 cells, but in Vero cells, pSFV-MD showed a similar level of reverse transcriptase activity, but a faster progression of CPE. HTS variant analysis of pSFV-MD and SFV-MD passaged through Vero cells showed accumulation of G-to-A mutations in the <i>bel2</i> and <i>tas</i> genes. Some of the mutations created stop codons, truncating Tas and Bet proteins. The generation of genomic variants in SFV-1 was identified by passaging the pSFV-MD genetically homogenous virus stock in Vero cells. The accumulation of APOBEC SNVs in the SFV-1 genome highlights the species-specific interactions of Bet and APOBEC proteins since the macaque SFV-1 Bet could not effectively counteract the AGM APOBEC activity in Vero cells.</p>

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Characterization of a synthetic infectious cloned DNA of simian foamy virus serotype 1

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月1日

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  • Sandra M. Fuentes,
  • Nicholas Mattson,
  • Trent J. Bosma,
  • Eunhae H. Bae,
  • Arifa S. Khan

摘要

A virus stock of a cloned infectious DNA of simian foamy virus serotype 1 (SFV-1) was obtained in M. dunni cells (designated as pSFV-MD). The kinetics of replication were similar to the parent uncloned SFV-MD stock in M. dunni cells and in FRhK-4 cells, but in Vero cells, pSFV-MD showed a similar level of reverse transcriptase activity, but a faster progression of CPE. HTS variant analysis of pSFV-MD and SFV-MD passaged through Vero cells showed accumulation of G-to-A mutations in the bel2 and tas genes. Some of the mutations created stop codons, truncating Tas and Bet proteins. The generation of genomic variants in SFV-1 was identified by passaging the pSFV-MD genetically homogenous virus stock in Vero cells. The accumulation of APOBEC SNVs in the SFV-1 genome highlights the species-specific interactions of Bet and APOBEC proteins since the macaque SFV-1 Bet could not effectively counteract the AGM APOBEC activity in Vero cells.