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<p>The rate of glucose import directly affects the maximum possible flux of central carbon metabolism. However, few tools can directly monitor the cellular glucose uptake rate. Here we report the development of a set of programmable bifunctional glucose uptake rate biosensors (GURBs) for real-time monitoring of glucose uptake rate, which enable the dynamic activation and inhibition of glucose uptake and central metabolism in <i>Escherichia coli</i>. These genetic circuits are used to monitor the glucose uptake rates of strains under different culture conditions. Also, feedback-loop control systems are designed to make cells rely on the glucose uptake rate to tune the target metabolic modules, resulting in a substantial increase of the titers of <span>l</span>-tryptophan, riboflavin and <span>d</span>-lactic acid. The glucose-uptake-rate-responsive genetic circuits developed in this study will serve as an effective tool for the dynamic control of glucose uptake and central metabolism.</p><p></p>

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Monitoring and dynamically controlling glucose uptake rate and central metabolism

  • 期刊论文

  • 发表日期：

    2025年1月17日

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  • Dongqin Ding,
  • Yaru Zhu,
  • Danyang Bai,
  • Tongxin Wan,
  • Sang Yup Lee,
  • Dawei Zhang

摘要

The rate of glucose import directly affects the maximum possible flux of central carbon metabolism. However, few tools can directly monitor the cellular glucose uptake rate. Here we report the development of a set of programmable bifunctional glucose uptake rate biosensors (GURBs) for real-time monitoring of glucose uptake rate, which enable the dynamic activation and inhibition of glucose uptake and central metabolism in Escherichia coli. These genetic circuits are used to monitor the glucose uptake rates of strains under different culture conditions. Also, feedback-loop control systems are designed to make cells rely on the glucose uptake rate to tune the target metabolic modules, resulting in a substantial increase of the titers of l-tryptophan, riboflavin and d-lactic acid. The glucose-uptake-rate-responsive genetic circuits developed in this study will serve as an effective tool for the dynamic control of glucose uptake and central metabolism.