Scholarlink

<p>Environmental dissemination of antimicrobial-resistant <i>Escherichia coli</i> is a major One Health challenge in Bangladesh. This study assessed the occurrence of third-generation cephalosporin and carbapenem-resistant <i>E. coli</i> across key environmental interfaces in Mymensingh. In May 2022, 28 water samples were collected from hospital wastewater, livestock effluents, aquaculture ponds, and the Khiro River. Samples were processed via the WHO Tricycle protocol, with 26 isolates undergoing whole-genome sequencing. Third-generation cephalosporin-resistant <i>E. coli</i> were detected in 86% of samples, while carbapenem-resistant isolates (18%) were found exclusively in hospital and river samples. Highest ESBL-producing <i>E. coli</i> concentrations occurred in hospital and poultry wastewater (mean 6.9–7.1 log10 CFU/ml). Sequencing identified 93 resistance genes, dominated by <i>bla</i>CTX-M-15 (79%), <i>tet(A)</i> (75%), <i>aadA1</i> (54%), <i>qnrS1</i> (50%), and <i>mph(A)</i> (50%). Carbapenem resistance was associated with <i>bla</i>NDM-5 in hospital and river isolates. While most isolates showed niche-specific clustering in our Phylogenetic analysis, highly conserved core-genome profiles (0 SNPs) between hospital and downstream river isolates (ST2363/ST410) provided strong genomic evidence consistent with effluent-mediated dissemination. These findings highlight the role of wastewater and livestock systems in AMR transmission, underscoring the urgent need for integrated One Health surveillance and improved waste management in Bangladesh.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Environmental dissemination of multidrug-resistant Escherichia coli across one health interfaces in Mymensingh, Bangladesh

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月27日

查看全文
  • Aninda Rahman,
  • Sangjukta Roy,
  • Nawroz Afreen,
  • Mohammad Gazi Shah Alam,
  • Mohammad Sadekuzzaman,
  • Abul Kalam,
  • Faisol Talukdar,
  • Md. Barkatul Alam,
  • Md. Shafiul Alam,
  • S. M. Sabrina Yesmin,
  • Anders Rhod Larsen,
  • Lina M. Cavaco,
  • Andreas Petersen,
  • Kazi Injamamul Hoque,
  • Mohammed Badrul Amin,
  • Rezaul Hasan,
  • Johanna Lindahl,
  • Jérôme Delamare-Deboutteville,
  • Arshnee Moodley

摘要

Environmental dissemination of antimicrobial-resistant Escherichia coli is a major One Health challenge in Bangladesh. This study assessed the occurrence of third-generation cephalosporin and carbapenem-resistant E. coli across key environmental interfaces in Mymensingh. In May 2022, 28 water samples were collected from hospital wastewater, livestock effluents, aquaculture ponds, and the Khiro River. Samples were processed via the WHO Tricycle protocol, with 26 isolates undergoing whole-genome sequencing. Third-generation cephalosporin-resistant E. coli were detected in 86% of samples, while carbapenem-resistant isolates (18%) were found exclusively in hospital and river samples. Highest ESBL-producing E. coli concentrations occurred in hospital and poultry wastewater (mean 6.9–7.1 log10 CFU/ml). Sequencing identified 93 resistance genes, dominated by blaCTX-M-15 (79%), tet(A) (75%), aadA1 (54%), qnrS1 (50%), and mph(A) (50%). Carbapenem resistance was associated with blaNDM-5 in hospital and river isolates. While most isolates showed niche-specific clustering in our Phylogenetic analysis, highly conserved core-genome profiles (0 SNPs) between hospital and downstream river isolates (ST2363/ST410) provided strong genomic evidence consistent with effluent-mediated dissemination. These findings highlight the role of wastewater and livestock systems in AMR transmission, underscoring the urgent need for integrated One Health surveillance and improved waste management in Bangladesh.